Biology subjects

Rogers, D.

Publications and source records attributed to Rogers, D..

21 records · Page 2Linked to original sources

bioRxiv2021

Pre-existing chromatin accessibility and gene expression differences among naïve CD4+ T cells influence effector potential

CD4+ T cells have a remarkable potential to differentiate into diverse effector lineages following activation. Here, we probed the heterogeneity present among naive CD4+ T cells before encountering their cognate antigen to ask whether their effector potential is modulated by pre-existing transcriptional and epigenetic differences. Using single-cell RNA sequencing, we showed that key drivers of variability are genes involved in T cell receptor (TCR) signaling. Using CD5 expression as a read-out of the strength of tonic TCR interactions with self-peptide MHC, and sorting on the ends of this self-reactivity spectrum, we find that pre-existing transcriptional differences among naive CD4+ T cells impact follicular helper cell (TFH) versus non-TFH effector lineage choice. Moreover, our data implicate TCR signal strength during thymic development in establishing differences in naive CD4 T cell chromatin landscapes that ultimately shape their effector potential.

immunology↗

bioRxiv2020

Asymmetrical pegs in square holes? Functional and phylogenetic determinants of plant community assembly in temperate forest understories

Despite advances in community assembly theory, uncertainties remain regarding how ecological and evolutionary processes shape species distributions and communities. We analyzed patterns of occurrence for 139 herbaceous plant species across 257 forest stands in Wisconsin (USA) to test predictions from community assembly theory. Specifically, we applied Bayesian phylogenetic linear mixed effects models (PGLMMs) to examine how functional traits and phylogenetic relationships influence plant distributions along environmental gradients and how functional similarity and phylogenetic relatedness affect local species co-occurrence. Leaf height, specific leaf area, and seed mass mediate species distributions along edaphic, climatic, and light gradients. In contrast, functional trait similarity and phylogenetic relationships only weakly affect patterns of local co-occurrence. These results confirm that broad-scale plant distributions are largely shaped by ecological sorting along environmental gradients but suggest deterministic assembly rules based on niche differentiation and complementary resource use may not govern local species co-occurrence in homogeneous environments. Statement of authorshipJB conceived the idea for the study. DL, SJ, and DR collected the vegetation and functional trait data. JB analyzed the data with assistance from DL. KC, KS, TG, and DW secured funding for research and oversaw data collection. JB wrote the first draft of the manuscript, all authors contributed to manuscript revisions. Data accessibility statementUpon acceptance, data will be archived at Figshare (https://figshare.com/) and scripts used to analyze the data will be shared on Github (https://github.com/jaredjbeck/).

ecology↗

bioRxiv2018

Fatigue induces long lasting detrimental changes in motor skill learning

Fatigue due to physical exertion is a ubiquitous phenomenon in everyday life and especially common in a range of neurological diseases. While the effect of fatigue on limiting skill execution are well known, its influence on learning new skills is unclear. This is of particular interest as it is common practice to train athletes, musicians or perform rehabilitation exercises up to and beyond a point of fatigue. In a series of experiments, we describe how fatigue impairs motor skill learning beyond its effects on task execution. The negative effects on learning are evidenced by impaired task acquisition on subsequent practice days even in the absence of fatigue. Further, we found that this effect is in part mediated centrally and can be alleviated by altering motor cortex function. Thus, the common practice of training while, or beyond, fatigue levels should be carefully reconsidered, since this affects overall long-term skill learning.

neuroscience↗