Biology subjects

Rimon, A.

Publications and source records attributed to Rimon, A..

3 recordsLinked to original sources

bioRxiv2024

Complete Genome Sequence of Six Pseudomonas aeruginosa Bacteriophages aim for research and treatments

IntroductionPseudomonas aeruginosa is a major opportunistic pathogen associated with healthcare-associated infections. The rise of antibiotic-resistant strains necessitates alternative treatment strategies, with bacteriophage therapy being a promising approach. MethodsSix bacteriophages were isolated from sewage samples. Phage isolation involved centrifugation, filtration, and plaque assays. The morphology of each was examined using Transmission Electron Microscopy (TEM). Genomic DNA was sequenced analyzed and compared. Phages lytic activity was assessed using growth curve analysis. ResultsThe six phages displayed distinct genomic and morphological characteristics, in three genomic clusters. No known virulence or antibiotic resistance genes were detected, indicating their safety for therapeutic use. TEM analysis revealed diverse morphologies, with some phages belonging to the Siphoviridae family and others to the Myoviridae family. Lysogenic phages demonstrated less effective lytic activity. ConclusionSome of these phages are promising candidates for the research of phage therapy efficacy, and the lytic phages can be used against P. aeruginosa infections.

microbiology↗

bioRxiv2024

Pulsed blue light and phage therapy: A novel synergistic bactericide

Antibiotic-resistant Pseudomonas aeruginosa (PA) is a critical health threat. Novel treatment approaches are urgently required in this post-antibiotic era. In the current study, we investigated the bactericidal combinatorial potential of two non-antibiotic alternative approaches: phage therapy and pulsed blue light (PBL). Bacteriophages (phages), are viruses that specifically infect and lyse bacteria without harming eukaryotic cells. Pulsed blue light (PBL) alters bacterial membranes and was clinically shown to be innocuous to the skin in low doses. Here, using a low dose 457nm, 33KHz PBL combined with specific PA targeting phages, we demonstrated a synergistic effect that achieved complete inhibition of planktonic bacteria and a 40% reduction in formed biofilms. As part of this study, we also developed a user-friendly python-based tool for extraction of growth curve outcomes. In vivo studies are warranted for further validation of this combinatorial treatment. This approach may lead to a novel, antibiotic complementary modality to help patients suffering from difficult-to-treat antibiotic-resistant infections. One Sentence SummaryLow-dose pulsed blue light and phage therapy have a synergistic bactericidal effect on Pseudomonas aeruginosa planktonic cultures and formed biofilm

microbiology↗

bioRxiv2022

Towards phage therapy for acne vulgaris: Topical application in a mouse model

Acne vulgaris is a common neutrophile-driven inflammatory skin disorder in which Cutibacterium acnes (C. acnes) bacteria play a significant role. Until now, antibiotics have been widely used to treat acne vulgaris, with the inevitable increase in bacterial antibiotic resistance. Phage therapy is a promising solution to the rising problem of antibiotic-resistant bacteria, utilizing viruses that specifically lyse bacteria. Here, we explored the feasibility of phage therapy against C. acnes. By combining eight novel phages we had isolated, together with commonly used antibiotics, we achieved 100% eradication of clinically isolated C. acnes strains. Using topical phage therapy in an acne mouse model resulted in significantly superior clinical scores, as well as a reduction in neutrophil infiltration compared to the control group. These results demonstrate the potential of phage therapy in acne vulgaris treatment, especially when antibiotic-resistant strains are involved.

microbiology↗