Biology subjects

Ricachenevsky, F.

Publications and source records attributed to Ricachenevsky, F..

3 recordsLinked to original sources

bioRxiv2020

Introgression from Oryza meridionalis into domesticated rice Oryza sativa results in shoot-based iron tolerance

Iron (Fe) toxicity is one of the most common mineral disorders affecting rice (Oryza sativa) production in flooded lowland fields. Oryza meridionalis is endemic from Northern Australia and grows in regions with Fe rich soils, making it a candidate for use in adaptive breeding. Aiming to understand tolerance mechanisms in rice, we screened a population of interspecific introgression lines (IL) from a cross between O. sativa and O. meridionalis for the identification of QTLs contributing to Fe toxicity tolerance. Six putative QTLs were identified. A line carrying one introgression from O. meridionalis on chromosome 9 associated with one QTL was highly tolerant despite very high shoot Fe concentrations. Physiological, biochemical, ionomic and transcriptomic analyses showed that the IL tolerance could partly be explained by Fe retention in the leaf sheath and culm. We constructed the interspecific hybrid genome in silico for transcriptomic analysis, and identified differentially regulated introgressed genes from O. meridionalis that could be involved in shoot-based Fe tolerance, such as metallothioneins, glutathione S-transferases and transporters from ABC and MFS families. This is the first work to demonstrate that introgressions of O. meridionalis into the O. sativa genome can confer increased tolerance to excess Fe. HighlightWe identified QTLs associated with iron tolerance derived from O. meridionalis, and characterized their physiological basis in O. sativa.

plant biology↗

bioRxiv2018

A curated list of genes that control elemental accumulation in plants.

Understanding the mechanisms underlying plants adaptation to their environment will require knowledge of the genes and alleles underlying elemental composition. Modern genetics is capable of quickly, and cheaply indicating which regions of DNA are associated with particular phenotypes in question, but most genes remain poorly annotated, hindering the identification of candidate genes. To help identify candidate genes underlying elemental accumulations, we have created the known ionome gene (KIG) list: a curated collection of genes experimentally shown to change uptake, accumulation, and distribution of elements. We have also created an automated computational pipeline to generate lists of KIG orthologs in other plant species using the PhytoMine database. The current version of KIG consists of 176 known genes covering 5 species, 23 elements and their 1588 orthologs in 10 species. Analysis of the known genes demonstrated that most were identified in the model plant Arabidopsis thaliana, and that transporter coding genes and genes altering the accumulation of iron and zinc are overrepresented in the current list.

plant biology↗

bioRxiv2018

Unraveling rice tolerance mechanisms against Schizotetranychus oryzae mite infestation

Infestation of Schizotetranychus oryzae (Acari: Tetranychidae) causes great losses in rice productivity. Infestation in Puita INTA-CL cultivar reduced the number of seeds/plant, percentage of full seeds, 1,000 seeds weight, and seed length, whereas infestation in IRGA423 increased 1,000 seeds weight and seed length. Reduction in seed weight/plant caused by infestation was higher in Puita INTA-CL than IRGA423. Thus, Puita INTA-CL was established as susceptible, and IRGA423 as tolerant to S. oryzae infestation. Photosynthetic parameters were less affected by infestation in IRGA423 than in Puita INTA-CL. Infestation also caused accumulation of H2O2, decreased cell membrane integrity and accelerated senescence in leaves of Puita INTA-CL, while leaves of IRGA423 presented higher levels of phenolics compounds. Using proteomic analysis, we identified proteins related to plant defense, such as jasmonate synthesis, and related to other mechanisms of tolerance such as oxidative stress, photosynthesis, and DNA structure maintenance, more abundant in IRGA423 after seven days of infestation. We detected higher levels of silicon (as amorphous silica cells) in leaves of infested IRGA423 plants compared to Puita INTA-CL, an element previously linked to plant defense. Our data shows that IRGA423 presents tolerance to S. oryzae infestation, and that multiple mechanisms might be employed by this cultivar.\n\nHighlightThis is the first report evaluating the defense responses (tolerance and susceptiblity) of two contrasting rice cultivars to Schizotetranychus oryzae mite infestation.

plant biology↗