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Biology subjects

Recco, D. P.

Publications and source records attributed to Recco, D. P..

2 recordsLinked to original sources

The Role of Secondary Chordae Tendineae in Mitral Valve Mechanics: A Benchtop Study

PurposeSecondary chordae tendineae (CT) are frequently disregarded during mitral valve (MV) repair. However, their contribution to MV biomechanics remains poorly understood. We hypothesized that secondary CT play an important role in maintaining leaflet geometry during valve closure. MethodsEleven explanted porcine MVs were micro-CT scanned in a systolic configuration under three conditions: (1) physiological baseline with all secondary CT intact, (2) transection of the A2 strut CT, and (3) transection of all secondary CT. Billowing height (BH), coaptation height (CH) along the parasternal long-axis view, and coaptation area (CA) were quantified and compared between conditions. An increase in BH[≥] 2 mm from baseline was defined as pathological. ResultsTransection of the A2 strut CT increased BH by 3.1 mm, exceeding the pathological billowing threshold by 1.1 mm (padj = 0.003). CH decreased by 0.6 mm (padj = 0.003), whereas CA showed no significant change compared with baseline. Subsequent transection of all secondary CT further increased BH to 4.8 mm relative to baseline, corresponding to 2.8 mm above the billowing threshold (padj = 0.003). CH did not differ significantly, while CA decreased by 0.35 cm2 compared with baseline (padj = 0.029). All values represent medians. ConclusionSecondary CT play a critical role in stabilizing MV leaflet geometry. Their transection resulted in increased leaflet billowing and reduced coaptation metrics associated with unfavorable MV repair outcomes. Preservation or reconstruction of secondary CT may therefore be an important consideration in MV repair strategies.

bioengineering↗

Parametric Engineering of Atrioventricular Living Valve Transplants

Diseases of the (mitral and tricuspid) atrioventricular valves (AVV), which regulate inflow from the atria to the ventricles, can result in severe obstruction to inflow (stenosis) or valvular leakage (regurgitation), requiring surgical intervention. In patients with small annulus diameters (< 19 mm), valve replacement is a clinical challenge limited by prosthesis size constraints, lack of growth potential, suboptimal durability, and elevated thrombosis and bleeding risk. While living valve transplantation (LVT) has re-opened the possibility of using allogeneic valve tissue capable of growth and remodeling, translating this to the AVV has been challenging given the anatomical complexity of the sub-valvular apparatus. Here, we propose a strategy using a replacement bi-leaflet cylindrical valve fabricated from donor AVV tissue and artificial chordae, with a geometry designed to mimic the native AVV and engineered to satisfy predefined clinical targets. Pulse duplicator experiments allowed characterization of valve dynamics in terms of clinically important attributes framed as dimensionless parameters. A multi-objective optimization allowed us to identify an optimal design which we implemented in porcine AVV replacements (n=6). Our results demonstrated favorable hemodynamics with minimal regurgitation and stenosis, suggesting a promising method for patient-optimized valve replacements.

bioengineering↗