Biology subjects

Ratnaparkhi, A.

Publications and source records attributed to Ratnaparkhi, A..

6 recordsLinked to original sources

bioRxiv2026

Glial ceramide orchestrates Lipid Droplet homeostasis and age-dependent motor function in Drosophila

Glia cells have emerged as equal partners to neurons in the development and maintenance of the nervous system. In this study, we examine the roles of ceramide metabolism and intracellular transfer in glia, contrasting these with those of neurons. We find that glia are more sensitive to the cessation of ceramide synthesis than neurons. Knockdown of ER-localised ceramide synthesis enzymes in glia, but not in neurons, leads to age-dependent motor defects. Intriguingly, Lipid Droplet (LD) size and density are lowered when either glial ceramide is reduced or its transfer to the Golgi, by Ceramide Transfer protein (CERT), is disturbed. Glial CERT knockdown or disruption of its interaction with the ER tethering protein VAPB also affects both motor function and LD dynamics, highlighting the importance of targeted, efficient non-vesicular ceramide transfer at membrane contact sites. Our research implicates reduced flux through the sphingolipid pathway in glial cells as a critical determinant of adult motor function, with LDs serving as a sensitive diagnostic readout. As such, our study has implications for a host of human motor neuron diseases that show late-onset motor deficits. Summary StatementWe find that the production and transfer of ceramides in glial cells are required for both Lipid Droplet formation and maintenance of adult motor function.

neuroscience↗

bioRxiv2025

Mon1-Rab7 axis is essential for transport, localization and anchoring of oskar mRNA

Asymmetric localization of oskar mRNA to the posterior of the oocyte is a complex process driven by autonomous and non-cell autonomous mechanisms. The former includes Oskar protein that reinforces localization and anchoring of its mRNA through activation of endocytosis and regulation of actin cytoskeleton; the latter includes signals from the posterior follicle cells (PFCs) that regulates microtubule orientation for polarized transport. Here we identify Monensin Sensitivity 1 (Mon1), as a novel factor regulating anterior-posterior (A-P) patterning. Mon1 is an evolutionarily conserved activator of Rab7-a key regulator of the endo-lysosomal pathway. Embryos lacking maternal mon1 (mon1m) show mislocalized oskar and bicoid mRNAs leading to loss of patterning and lethality. In the mutant oocyte Staufen appears clumpy and the levels of Oskar protein and Par-1 is significantly reduced. Abnormal actin rings are seen in the ooplasm. Driving expression of mon1 in the germline rescues these phenotypes and restores viability. In contrast, expression in the PFC predominantly rescues the Par-1 phenotype with a modest effect on viability. We demonstrate that oskar mRNA interacts with Rab7 suggesting possible role for the Mon1-Rab7 axis in the transport of oskar. We show that Mon1 in the PFCs, regulates PIP2 levels to influence accumulation of Par-1 in the oocyte. We propose that Mon1 regulates oskar localization in two distinct ways: cell autonomously in the germline by regulating Rab7, and non-cell autonomously through the PFCs by regulating accumulation of Par-1. Summary statementMon1, an established Rab converter, has roles in embryonic axial patterning, modulating transport, localisation and anchoring of posteriorly localised mRNA during oocyte maturation. Mon1 influence is both cell autonomous, from within the oocyte and non-cell autonomous, through posterior follicle cells.

developmental biology↗

bioRxiv2021

Caspar, an adapter for VAP and TER94, delays the progression of disease by regulating glial inflammation in a Drosophila model of ALS8

Amyotrophic Lateral Sclerosis (ALS) is a fatal, late onset, progressive motor neurodegenerative disorder. We have been studying cellular and molecular mechanisms involved in ALS using a vesicle-associated membrane protein-associated protein B (VAPB/ALS8) Drosophila model, which mimics many systemic aspects of the human disease. Here, we show that the ER resident VAPB interacts with Caspar, an ortholog of human fas associated factor 1 (FAF1). Caspar, in turn, interacts with transitional endoplasmic reticulum ATPase (TER94), a fly ortholog of ALS14 (VCP/p97, Valosin-containing protein), via its UBX domain and poly-ubiqutinated proteins with its UBA domain. Caspar overexpression in the glia extends lifespan and also slows the progression of motor dysfunction in the ALS8 model, a phenomenon that we ascribe to its ability to restrain age-dependant inflammation, modulated by Relish/NF{kappa}B signalling. We hypothesize that Caspar is a key molecule in the pathogenesis of ALS. Caspar connects the plasma membrane (PM) localized immune signalosome to the ER based VAPB degradative machinery, presumably at PM:ER contact sites. The Caspar:TER94:VAPB complex appears to be a strong candidate for regulating both protein homeostasis and NF{kappa}B signalling. These, in turn, regulate glial inflammation and determine progression of disease. Our study projects human FAF1 as an important protein target to alleviate the progression of motor neuron disease.

genetics↗

bioRxiv2020

Dmon1 and rab7 interact to regulate glutamate receptor GluRIIA levels at the larval Drosophila neuromuscular junction.

Regulation of post-synaptic receptors plays an important role in determining synaptic strength and plasticity. The Drosophila larval neuromuscular junction (nmj) has been used extensively as a model to understand some of these processes. In this context, we are interested in the role of Drosophila Monensin sensitive protein 1 (DMon1) in regulating glutamate receptor (GluRIIA) levels at the nmj. Dmon1 is an evolutionarily conserved protein which, in complex with CCZ1, regulates the conversion of early endosomes to late endosomes through recruitment of Rab7. C-terminal deletion mutants of Dmon1 (Dmon1{Delta}181) exhibit lethality. The escapers have a short life span and exhibit severe motor defects. At the nmj, these mutants show a defects in synaptic morphology and a strong increase in glutamate receptor GluRIIA levels. The mechanism by which Dmon1 regulates GluRIIA is unclear. In this study, we have described the characterization the mutation in an EMS mutant referred to as pog1 and demonstrate this mutant to be an allele of Dmon1. Further, we have examined the role of rab7 in regulation the of GluRIIA. We show that similar to Dmon1, knock-down of rab7 using RNAi in neurons, and not muscles, leads to an increase in GluRIIA. Loss of one copy each of Dmon1 with rab7 leads to a synergistic increase in receptor expression. Further, overexpression of an activated Rab7 can rescue the GluRIIA phenotype observed in Dmon1{Delta}181 mutants. Together, these results highlight a neuronal role for Rab7 in GluRIIA regulation and underscores the important of the endo-lysosomal pathway in this process.

genetics↗

bioRxiv2019

FGFR/Heartless and Smog interact synergistically to negatively regulate Fog mediated GPCR signaling.

G-protein coupled receptor (GPCR) signaling triggered by Folded gastrulation (Fog) is one of the pathways known to regulate glial organization and morphogenesis in the embryonic CNS in Drosophila. Fog is best known for its role in epithelial morphogenesis during gastrulation. Here, the signaling pathway includes GPCRs Mist and Smog and the G-Protein Concertina (Cta) which activate downstream effectors to bring about cytoskeletal changes essential for cell shape change In this study, we identify molecular players that mediate and serve as important regulators of Fog signaling in the embryonic CNS. We find that while Cta is essential for Fog signaling neither receptors, Mist nor Smog mediates signaling in the CNS. On the contrary, we find that Smog functions as a negative regulator of the pathway. Surprisingly, Heartless which encodes a fibroblast growth factor receptor, also functions as a negative regulator of Fog signaling. Further, we find that both heartless and smog interact in a synergistic manner to regulate Fog signaling. This study thus identifies novel regulators of Fog signaling that may play an important role in fine-tuning the pathway to control cell morphogenesis. It also suggests the likelihood of there being multiple receptors for Fog that mediate and regulate signaling in a context specific manner. Author SummaryIn Drosophila, Folded gastrulation (Fog) functions as ligand that signals via GPCRs to regulate cell shape during gastrulation -one of the earliest events in embryogenesis. Here, Fog signals via receptors Mist and Smog to activate the G-protein Concertina to elicit change in cell shape. In the embryonic central nervous system (CNS) this pathway regulates shape and organization of glia important for functions such as insulation of neurons and synapses. The mechanism of Fog signal transduction in the CNS and its regulation is not well understood. We have sought to address these questions in our study. We find that Concertina is an essential factor for Fog signaling in the CNS but interestingly Mist is not. In contrast, Smog functions as a negative regulator such that loss of Smog enhances Fog signaling. A similar role is played by the receptor tyrosine kinase-Heartless. Interestingly, we find that Smog and Heartless interact as part of a common genetic network to regulate Fog signaling. Our results thus provide novel insights into the regulation of Fog signaling and shed light on how signaling can be fine-tuned in a context dependent manner to control cell shape change which plays a critical role during development and organ formation.

cell biology↗

bioRxiv2018

Rab converter DMon1 constitutes a novel node in the brain-gonad axis essential for female germline maturation

Monensin-sensitive 1 (Mon1) is an endocytic regulator that participates in the conversion of Rab5 positive early endosomes to Rab7 positive late endosomes. In Drosophila, loss of mon1 (Dmon1) leads to sterility. The Dmon1 mutant females have extremely small ovaries with complete absence of late stage egg chambers-a phenotype reminiscent of mutations in the insulin pathway genes. Consistently, we find that expression of many Drosophila insulin-like peptides (Dilps) is down regulated in Dmon1 mutants. Conversely, feeding an insulin-rich diet can rescue the ovarian defects induced by the loss of Dmon1.\n\nSurprisingly however, Dmon1 is required in the tyramine/octopaminergic neurons (OPNs) and not in the ovaries or the insulin producing cells (IPCs). Thus, knockdown of Dmon1 in just the OPNs is sufficient to mimic the ovarian phenotype while expression of Dmon1 in the OPNs alone, is sufficient to rescue the mutant defect.\n\nLastly, we have identified dilp5 as a critical target of Dmon1. Both, protein and mRNA levels of Dilp5 levels are reduced in Dmon1 mutants and IPC-specific dilp5 over expression can ameliorate the Dmon1 dependent sterility defect. The study thus identifies Dmon1 as a novel molecular player in the brain-gonad axis and underscores the significance of inter-organ systemic communication during development.\n\nSignificance Statement Functional significance of the long-distance systemic communication during organogenesis has emerged as a major area of enquiry. We have focused our attention on an endocytic regulator DMon1, that appears to participate in a remote control type of mechanism. We report a novel tripartite circuitry that involves Dmon1 activity in Octopaminergic neurons, its influence on insulin production in the insulin producing cells (IPCs) which, in turn, is required for the progression of oogenesis. Our results document a spatially remote non-autonomous control mechanism involving neuronal cross-talk that orchestrates developmental regulation of oogenesis. Importantly our data provide a unique example of how distinct neuronal hubs can engineer metabolic pathways underlying growth/differentiation and highlight the importance of systemic regulation of organogenesis.

developmental biology↗