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Biology subjects

Ramakrishnan, A. K.

Publications and source records attributed to Ramakrishnan, A. K..

2 recordsLinked to original sources

Spatial separation of Streptomyces aerial mycelium during fermentation enhances secondary metabolite production

Conditioning of morphology is an effective technique to enhance secondary metabolite production by Streptomyces. Here we report a novel conditioning method employing glass marbles in batch cultures to enhance secondary metabolite production by Streptomyces sp. The marbles seem to spatially separate aerial and submerged mycelia in the flask which was necessary for the qualitative and quantitative enhancement of metabolite production of secondary metabolites. The method also offers shorter incubation period compared to conventional methods for effective production. Further, using a combination of this method and response surface methodology we could enhance the production of antimycobacterial molecules chrysomycin A and B significantly. ImportanceRediscovery of existing molecules and lack of techniques to induce production of secondary metabolites are the major bottlenecks associated with drug discovery of novel bioactive molecules from Streptomyces, the major source of marketable drugs today. We found a new method to increase the diversity and quantity of secondary metabolites in two Streptomyces species. This method thus enhances the chance of finding novel active principles from Streptomyces.

microbiology↗

Putative TetR family transcriptional regulator Rv1019 of Mycobacterium tuberculosis is an auto-repressor and a negative regulator of mfd-mazG operon

Regulation of gene expression at the level of transcription is one of the ways living organisms differentially make proteins in their cells. Intracellular pathogen Mycobacterium tuberculosis persists in the host for years in a latent state in structures called granuloma. Rv1019, a member of an uncharacterized TetR family of transcriptional regulators of Mtb H37Rv, was found to be differentially expressed during dormancy and reactivation in vitro. By GFP reporter assays, electrophoretic mobility shift assays and chromatin immuno-precipitation assays we showed that this protein binds to its own promoter and acts as a negative regulator of its own expression. It forms dimers in vitro which is essential for the DNA binding activity, which is abrogated in the presence of tetracycline. We show that Rv1019 and downstream genes Rv1020 (mfd), Rv1021 (mazG) are co-transcribed. Constitutive expression of Rv1019 in M. smegmatis downregulated MSMEG_5423 (mfd) and MSMEG_5422 (mazG) suggesting that Rv1019 negatively regulates these downstream genes. M. smegmatis expressing Rv1019 was found to be sensitive to UV and H2O2 compared to the control suggesting its role in regulating DNA damage response in mycobacterium. ImportanceOne of the reasons for our inability to eradicate tuberculosis, in spite of the availability of effective drugs and BCG vaccine, is the ingenuity of Mycobacterium tuberculosis to survive, persist and multiply inside the lungs where it employs clever strategies to counter the host defence systems. The number of anti-TB drugs in the current therapeutic regimen is limited, and the long duration of treatment increases the chances for emergence of drug resistant bacteria. Rv1019, a transcriptional regulator differentially expressed in in vitro dormancy-reactivation studies, was found to be an auto-repressor and negatively regulating mfd-mazG operon involved in DNA repair. As Rv1019 is a regulator of critical pathways in bacterial persistence, it could be a prospective target for prophylactic intervention.

microbiology↗