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Rama, R.

Publications and source records attributed to Rama, R..

2 recordsLinked to original sources

Multimodal Study of the Effects of Varying Task Load Utilizing EEG, GSR and Eye-Tracking

ObjectiveThe effect of task load on performance is investigated by simultaneously collecting multi-modal physiological data and participant response data. Periodic response to a questionnaire is also obtained. The goal is to determine combinations of modalities that best serve as predictors of task performance.\n\nApproachA group of participants performed a computer-based visual search task mimicking postal code sorting. A five-digit number had to be assigned to one of six different non-overlapping numeric ranges. Trials were presented in blocks of progressively increasing task difficulty. The participants responses were collected simultaneously with 32 channels of electroencephalography (EEG) data, eye-tracking data, and Galvanic Skin Response (GSR) data. The NASA Task-Load-Index self-reporting instrument was administered at discrete time points in the experiment.\n\nMain resultsLow beta frequency EEG waves (12.5-18 Hz) were more prominent as cognitive task load increased, with most activity in frontal and parietal regions. These were accompanied by more frequent eye blinks and increased pupillary dilation. Blink duration correlated strongly with task performance. Phasic components of the GSR signal were related to cognitive workload, whereas tonic components indicated a more general state of arousal. Subjective data (NASA TLX) as reported by the participants showed an increase in frustration and mental workload. Based on one-way ANOVA, EEG and GSR provided the most reliable correlation to perceived workload level and were the most informative measures (taken together) for performance prediction.\n\nSignificanceNumerous modalities come into play during task-related activity. Many of these modalities can provide information on task performance when appropriately grouped. This study suggests that while EEG is a good predictor of task performance, additional modalities such as GSR increase the likelihood of more accurate predictions. Further, in controlled laboratory conditions, the most informative or minimum number of modalities can be isolated for monitoring in real work environments.

neuroscience

TRAF6 controls excitatory spinogenesis and excitation-inhibition balance thought binding neuroplastin

Synaptogenic mechanisms and their relevance to achieve a correct synapse density and activity in mature neurons are poorly understood. Here, we show that the tumor necrosis factor receptor-associated factor 6 (TRAF6) controls early spinogenesis by binding the cell adhesion molecule neuroplastin which is has been related to synapse formation in vivo. TRAF6-neuroplastin co-precipitations from brain samples and co-transfected HEK cells is explained by direct interaction of the proteins based on three-dimensional modelling and biochemical identification of intracellular amino acids of neuroplastin binding the TRAF-C domain of TRAF6 with micromolar affinity. TRAF6 was not only required for normal spinogenesis but also was strictly necessary to restore failed spinogenesis in neuroplastin-deficient neurons. Independently from neuroplastins extracellular adhesive properties or interaction with another known partner i.e. the plasma membrane Ca2+ ATPases, TRAF6 mediated formation of new postsynapses by neuroplastin overexpression in rat hippocampal neurons. Furthermore, TRAF6-controlled spinogenesis was required for the establishment of a correct synapse density as well as proper synaptic activity and intrinsic neuronal activity as demonstrated with intracellular and extracellular electrophysiological recordings. These findings provide a novel mechanism for early synapse formation that shapes connectivity and functioning of hippocampal neurons.

cell biology