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Raj, E.

Publications and source records attributed to Raj, E..

2 recordsLinked to original sources

Development of non-destructive methods to estimate functional traits and field evaluation in tea plantations using a smartphone

Smartphones are equipped with various types of sensors which make them a promising tool to assist diverse digital farming tasks because of their mobility, cost, accessibility, and computing power allow us to perform real-time practical applications. This paper presents the utilization of various non-destructive methods of nutrient and disease classification techniques using smartphone collected images, processed through various image segmentation algorithms. Both in vivo and in vitro estimations shows comparable results with both chlorophyll and nitrogen contents of a crop shoot. Moreover, the correlation between SPAD measured values and nitrogen of crop shoot showed a significant linear association (R2 =0.7309), revealing the potency of in vivo observation for prediction of actual chlorophyll content in tea crop. SPAD values and yield have a strong linear relationship (R2 =0.7103), in which SPAD-meter performed better detection at very low values. The study concluded that the proposed techniques could be used for automatic detection as well as classification of foliar diseases and nutrients in tea.

plant biology

Gene expression profiling of Tea (Camellia sinensis L.) in response to biotic stress using microarrays

The blister blight (BB) and grey blight (GB) diseases are the major biotic stresses, which affecting the plant health, yield and quality of tea. The study aims to understand the gene response of tea plants against destructing foliar diseases in terms of differential gene expression and their pathways through microarray analysis aid by MapMan(R) software. The results of expression profile analysis showed that 235 in BB and 258 for GB genes were differentially expressed (at P<0.05) which involving in gene regulatory function as biotic stress response. Similarly, 76 and 86 differentially expressed genes involving in cellular response during BB and GB diseases, respectively. However, 28 in BB and 9 in GB differentially expressed (P<0.01) genes were putatively involved in biotic stress response. The study also identified differentially expressed 75 transcription factors (TFs) belongs to 23 TFs superfamily act as either transcriptional activators or repressors. The study helps to understand the differential gene expression pattern and its cellular, molecular and biological mechanisms of tea plants of two different diseases based on microarray analysis. Further studies using biotechnological tools on the stress-responsive genes in the germplasm may enable us for development of disease resistance.

plant biology