Exploration of Antibacterial Activities of Berberis royleana Fractions Extracts
ObjectivesPrepare various solvent extracts of Berberis royleana (areal part of plant) to determine the in vitro antibacterial potential of methanolic, ethyl acetate, chloroform, n-hexane and water extracts of B. royleana against various bacterial isolates and Compare the efficacy of outstanding antimicrobial extracts of B. royleana with commonly used antibiotics. Berberis species are medicinally important plants, produce various metabolites and used as treatment for multiple complications. Berberis royleana is a rare specie belongs to genus Berberis. MethodsIn the current study the areal parts of the plant were isolated to explore antibacterial activities. Antibacterial activities were done using standard procedures. The antibacterial activities of different fractions were tested by 100 g methanolic, ethyl acetate, chloroform, n-hexane and water fractions of B. royleana against bacterial isolates Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella Typhi and Proteus spp. The ciprofloxacin (5g) was used as a positive control and DMSO as a negative control. ResultsAll fractions showed zone of inhibition against the growth of tested bacterial isolates. Methanolic fractions have maximum ZI against S. aureus and K. pneumoniae (25.7{+/-}1.5 mm), S. aureus (23{+/-}2.7), Salmonella Typhi (25{+/-}1), water fraction have Klebsiella pneumoniae (24.4 {+/-}2.5), Salmonella Typhi (23 {+/-}1 mm), S. aureus (21{+/-}2.8 mm) and the n-hexane fraction exhibits ZI against K. pneumoniae (24.7{+/-}1.5), Salmonella Typhi (24{+/-}2) S. aureus, ethyl acetate maximum zone against E. coli (16.6{+/-}3.1) and chloroform fraction showed maximum ZI against S. Typhi (21.6{+/-}3.3). ConclusionFrom current report it may be concluded that B. royleana extracts have medicinally effective potentials against drugs resistant bacteria.