Que, Y. | Search bioRxiv

bioRxiv2023

Transcription factor ScWRKY4 in sugarcane negatively regulates the resistance to pathogen infection through the JA signaling pathway

WRKY transcription factor, the transcriptional regulators unique to plants, plays an important role in plant defense response to pathogen infection. However, the disease resistance mechanism of WRKY gene in sugarcane remains unclear. Previously, we identified a ScWRKY4 gene, a member of class IIc of the WRKY gene family, from the sugarcane cultivar ROC22. This gene could be induced by the stresses of salicylic acid (SA) and methyl jasmonate (MeJA). Interestingly, the expression of the ScWRKY4 gene was down-regulated in smut-resistant sugarcane cultivars but up-regulated in smut-susceptible sugarcane cultivars under Sporisorium scitamineum stress. Besides, stable overexpression of the ScWRKY4 gene in Nicotiana benthamiana enhanced susceptibility to Fusarium solani var. coeruleum and caused the down-regulated expression of immune marker-related genes. Furthermore, transcriptome analysis indicated that, the expression of most JAZ genes was suppressed in plant signal transduction pathway. In addition, ScWRKY4 could interact with ScJAZ13 and repress the expression of ScJAZ13. We thus hypothesized that the ScWRKY4 gene was involved in the regulatory network of plant disease resistance, most probably through the JA signaling pathway. The present study depicted the molecular mechanism of the ScWRKY4 gene involved in sugarcane disease resistance and laid the foundation for the subsequent investigation. HighlightTransgenic plants overexpressing the ScWRKY4 gene negatively regulated resistance to pathogen by inhibiting the expression of the JAZ genes.

plant biology↗

bioRxiv2019

Mass Spectrometry based Metabolomics Deciphered Metabolic Reprogramming That Was Required for Biofilm formation in Uropathogenic Escherichia coli

Biofilm formation plays a key role in many bacteria causing infections, which mostly accounts for high-frequency infectious recurrence and antibiotics resistance. In this study, we sought to compare modified metabolism of biofilm and planktonic populations with UIT89, a predominant agent of urinary tract infection, by combining mass spectrometry based untargeted and targeted metabolomics methods, as well as cytological visualization, which enable us to identify the driven metabolites and associated metabolic pathways underlying biofilm formation. Surprisingly, our finding revealed distinct differences in both phenotypic morphology and metabolism between two patterns. Furthermore, we identified and characterized 38 differential metabolites and associated three metabolic pathways involving glycerolipid metabolism, amino acid metabolism and carbohydrate metabolism that were altered mostly during biofilm formation. This discovery in metabolic phenotyping permitted biofilm formation shall provide us a novel insight into the desperation of biofilm, which enable to develop novel biofilm based treatments against pathogen causing infections, with lower antibiotic resistance.

microbiology↗