Biology subjects

Qamar, R.

Publications and source records attributed to Qamar, R..

3 recordsLinked to original sources

bioRxiv2020

Cotton growth, yield, quality and boron distribution as affected by soil-applied boron in calcareous saline soil

Boron (B) is deficient in the calcareous, Typic Haplocambid soils of cotton growing belt of Pakistan, and thus is a vital reason for less cotton yield in the region. In order to investigate the growth and quality alterations associated with soil applied B on cotton (cv. CIM-616 and CIM-600) an experiment was conducted. Boron was applied at 0.00, 2.60, 5.52, 7.78 and 10.04 mg B kg-1 of soil using borax (Na2B4O7.10H2O), in a complete randomized design with factorial arrangement with four replications. Results revealed that soil applied B @ 2.60 mg B kg-1 of soil significantly (P[≤]0.05) improved cotton growth, yield, quality and B distribution among different parts. Different growth and yield parameters like plant height, leaf area, number of bolls, boll size and weight, seed cotton yield, photosynthesis, transpiration rate, stomatal conductance, water use efficiency, GOT, staple length and fiber fineness and strength except B uptake by roots, seed, leaves and stalk plant body which was significantly increased with B (10.04 mg B kg-1) in both cultivars of cotton, but the degree of effects was varied between cultivars. The results indicated that studied traits of both cultivars were significantly (P[≤]0.05) decreased in B-deficient stressed treatments. Between hybrids, CIM-600 produced significantly (P[≤]0.05) maximum recorded parameters under 2.60 mg B kg-1 application compared than CIM-616. Our findings confirm that the adequate level of B (2.60 mg B kg-1) had pronounced effects on various growth, yield, physiological and fiber quality associated traits, as compared to B uptake traits of cotton cultivars.

plant biology↗

bioRxiv2019

Animal model and therapeutic prospects for left ventricular hypertrophy and associated renal complication

Cardiac and renal dysfunction often co-occur and considerably worsen the prognosis leading to difficulty in therapy in left ventricular hypertrophy (LVH). The aim of this study was to elucidate changes in expression of human ortholog genes of hypertension, vascular and cardiac remodeling and hypertensive nephropathy phenotypes under normal, disease and gasotransmitter, H2S (hydrogen sulphide) and NO (nitric oxide) and combined (H2S+NO), treatment of rat myocardium and renal tissues. LVH rat models were generated and were treated with H2S and NO. Relative gene expression was quantified. Heart and renal physical indices were significantly modified under individual as well as combined H2S+NO treatment in control and LVH rats. Expression analysis revealed, hypertension, vascular remodeling genes ACE, TNF and IGF1, mRNAs to be significantly increased (P<0.05) in myocardia and kidneys of LVH rats, while individual and combined H2S+NO treatment reduced gene expression to normal/near to normal values. The cardiac remodeling genes MYH7, TGF{beta}, SMAD4 and BRG1 expression were significantly up-regulated (P<0.05) in the myocardia of LVH and combined H2S+NO treatment, which recovered the normal/near to normal expression more effectively as compared to individual treatments. Interestingly, individual as well as combined H2S and NO treatment significantly decreased PKD1 expression in renal tissue, which was significantly up-regulated in LVH rats (P<0.05). The reduction in hemodynamic parameters and cardiac indices as well as alteration in gene expression on treatment in LVH rat model indicates important therapeutic potential of combined treatment with H2S+NO gasotransmitters in hypertension and cardiac hypertrophy associated with renal complications.

pharmacology and toxicology↗

bioRxiv2016

Mapping Autosomal Recessive Intellectual Disability: Combined Microarray and Exome Sequencing Identifies 26 Novel Candidate Genes in 192 Consanguineous Families

Approximately 1% of the global population is affected by intellectual disability (ID), and the majority receive no molecular diagnosis. Previous studies have indicated high levels of genetic heterogeneity, with estimates of more than 2500 autosomal ID genes, the majority of which are autosomal recessive (AR). Here, we combined microarray genotyping, homozygosity-by-descent (HBD) mapping, copy number variation (CNV) analysis, and whole exome sequencing (WES) to identify disease genes/mutations in 192 multiplex Pakistani and Iranian consanguineous families with non-syndromic ID. We identified definite or candidate mutations (or CNVs) in 51% of families in 72 different genes, including 26 not previously reported for ARID. The new ARID genes include nine with loss-of-function mutations (ABI2, MAPK8, MPDZ, PIDD1, SLAIN1, TBC1D23, TRAPPC6B, UBA7, and USP44), and missense mutations include the first reports of variants in BDNF or TET1 associated with ID. The genes identified also showed overlap with de novo gene sets for other neuropsychiatric disorders. Transcriptional studies showed prominent expression in the prenatal brain. The high yield of AR mutations for ID indicated that this approach has excellent clinical potential and should inform clinical diagnostics, including clinical whole exome and genome sequencing, for populations in which consanguinity is common. As with other AR disorders, the relevance will also apply to outbred populations.

genetics↗