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Pozniak, M.

Publications and source records attributed to Pozniak, M..

2 recordsLinked to original sources

DepoCat: Interactive database of experimentally verified phage depolymerases

Klebsiella phage depolymerases degrade polysaccharide capsules and exhibit narrow substrate specificity for particular capsular types. Despite a growing number of experimentally characterized enzymes, these data remain scattered throughout the scientific literature, while existing protein sequence repositories are dominated by entries with computationally assigned, unverified functional annotations. Here we present DepoCat, the first interactive database of phage depolymerases with experimentally verified function and specificity, available at http://depocat.uwr.edu.pl. The database currently contains 131 proteins meeting rigorous inclusion criteria, spanning 75 distinct capsular types. Each entry integrates experimental and computational resources. The web interface provides an integrated Classifier tool with two search modes: sequence-based search and structure-based search - enabling preliminary structural classification and inference of putative substrate specificity of newly identified depolymerases. We demonstrated the utility of both modes on a set of 17 experimentally verified non-Klebsiella phage depolymerases, for which structural analysis enabled unambiguous class assignment in almost all cases despite low or undetectable sequence similarity to the database reference dataset. DepoCat constitutes a publicly accessible resource supporting research into the structural diversity and sequence-structure-specificity relationships of phage depolymerases, while also facilitating the identification of candidates for therapeutic and diagnostic applications.

microbiology↗

DepoCatalog: Mapping the Diversity of 105 Recombinant Klebsiella Phage Depolymerases Across Sequence, Structure, and Substrate Specificity

A validated catalog of 105 recombinant depolymerases from Klebsiella phages covers 58 KL-types. 46 novel enzymes from prophages, jumbo phages, and common phages are linked to any known enzymatic activity against 14 classical serotypes and 12 genome-defined KL-types. Using activity-based profiling, structure prediction, and domain dissection, we developed a function-guided classification and a five-class structural catalog. This framework reveals highly specific enzymes active against up to three capsule types. K47 CPS was degraded by three diverse protein groups. Structurally similar depolymerases degrading particular CPS were found in distinct phage taxa, with highly conserved enzymes in Drulisvirus specific to K1-, K2-types. The exclusive depolymerases were found in siphoviruses targeting K2 and K62 serotypes. A case study of five structurally similar enzymes degrading KL22/KL37/KL111 and KL25/KL119 capsules suggested specificity switching via amino acid changes or C-domain modification. Klebsiella phage depolymerases catalog sheds light on their diversity, evolution, and potential application.

microbiology↗