Cryogenic non-invasive 3D X-ray phase-contrast imaging of unfixed, intact mouse joints reveals shifting chondrocyte hypertrophy across the endochondral interface
Objectivesi) develop and use a new cryogenically-enhanced phase contrast method to visualise hyaline articular cartilage (HAC); ii) to measure HAC, articular calcified cartilage (ACC) and total articular cartilage thicknesses in male STR/Ort (osteoarthritis, OA) and CBA (healthy) mouse tibial epiphyses, reflecting divergent OA predisposition, at three age timepoints chosen to reflect pre-OA, OA onset and late-progression; iii) to compare HAC, trans-zonal and ACC 3D chondrocyte anatomy in tibial epiphyses. MethodsSTR/Ort and CBA mouse knees (n=4 per age and strain group) were synchrotron-CT scanned at high-resolution while fresh frozen, without staining, fixation, dissection or dehydration of the joint capsule. Both cartilage thickness and cellular characteristics (chondrocyte n=420) were manually measured and statistically compared (SPSS). ResultsCryo-enhanced phase contrast allowed cartilage to be seen in full thickness with cellular detail. HAC was thicker in STR/Ort than age-matched CBA mice in 16/24 knee joint compartments and timepoints (all p<0.04). In contrast, HAC was thicker only in the posterior lateral femur of CBA mice at 10weeks (p<0.001, Table 1). ACC and total cartilage were also thicker in STR/Orts. Trans-zonal chondrocytes were smaller than ACC and HAC chondrocytes (p-values<0.001, volumes 878, 1,567m3 and 1,348m3 respectively). O_TBL View this table: org.highwire.dtl.DTLVardef@191d5a9org.highwire.dtl.DTLVardef@162523borg.highwire.dtl.DTLVardef@4b7caorg.highwire.dtl.DTLVardef@1be6005org.highwire.dtl.DTLVardef@192143d_HPS_FORMAT_FIGEXP M_TBL O_FLOATNOTABLE 1:C_FLOATNO O_TABLECAPTIONCondylar compartments in which HAC thickness was measured, and the presence or absence of a significant difference between STR/Ort and CBA mouse strains. * indicates a significant difference between strains (p<0.05). Note that the posterior lateral femur at 10 weeks of age is the only region in which STR/Ort mice have significantly thinner HAC than CBA mice. C_TABLECAPTION C_TBL ConclusionsCryogenically-enhanced phase-contrast imaging allowed cellular detail to be seen in 3D as never before in HAC in this (or any other) model. Our findings challenge current understanding by associating STR/Ort OA vulnerability with regions of thick, rather than thinning-with-age, cartilage. Our data affirm an association between excessively hypertrophic chondrocytes and OA is present in STR/Ort mice.