Search bioRxiv⌕ Search

Biology subjects

Polonini, H.

Publications and source records attributed to Polonini, H..

2 recordsLinked to original sources
bioRxiv2020

Characterization and safety profile of Imuno TF(R), a nutritional supplement for immune system regulation containing transfer factors

Imuno TF(R) is a nutritional supplement composed of isolated transfer factors (TF) from porcine spleen. It is composed of a specific mixture of molecules that impact functions of the biological systems, and historically is linked to the immune system regulation. In this study, we demonstrate for the first time its proteomic analysis, nutritional composition, and safety profile in terms of mutagenic potential and acute oral dose (LD50). The obtained analysis indicated the product is a complex set of oligo- and polypeptides constituted of 163 different peptides which can potentially act on multiple mechanisms on the immune system pathways. The chemical composition showed low fat and low sugar content, saturated fatty acids-free and the presence of 10 vitamins and 11 minerals. No mutagenic effect was observed, and the LD50 was 5,000 mg kg-1 body weight. This accounts for a safe product to be used by oral route, with potential benefits for the immune system.

immunology↗
bioRxiv2020

Immunoregulatory effects of Imuno TF (transfer factors) on Th1/Th2/Th17/Treg cytokines

Transfer factors are known since 1955 due to their activities on the immune system. Although the reports on the effects on diverse immune mechanisms, their role on Th1, Th2, Th17 and Treg responses was still not described. In this sense, the present work focused on the evaluation of such immune responses. For that, human lymphocytes, and mice thymic, splenic and Peyers cells were stimulated with Lipopolysaccharides and Concanavalin A, and then treated with isolated transfer factors (Imuno TF(R)). The culture medium was harvested and the quantification of Th1 cytokines (IL-2 and IFN-{gamma}), Th2 cytokines (IL-4, IL-5, and IL-13), Th17 cytokine (IL- 17), Treg cytokine (IL-35), inflammatory cytokines (IL-6 and TNF-), and anti-inflammatory cytokine (IL-10) was performed, as well as the quantification of mRNA levels. Imuno TF(R) positively regulated Th1 cytokines, while decreased Th2 cytokines. It also increased levels of mRNA and secretion of the anti-inflammatory cytokine IL-10, whereas it reduced levels of mRNA and the secretion of pro-inflammatory cytokines IL-6 and TNF-. Finally, it reversed the hypersecretion of IL-17 and did not promote significant changes in IL-35 secretion. This highlights the role of Imuno TF(R) in the regulation of the immune responses.

immunology↗