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Pinedo, P.

Publications and source records attributed to Pinedo, P..

3 recordsLinked to original sources

Evaluating machine learning algorithms to predict lameness in dairy cattle

Dairy cattle lameness represents one of the common concerns in intensive and commercial dairy farms. Lameness is characterized by gait-related behavioral changes in cows and multiple approaches are being utilized to associate these changes with lameness conditions including data from accelerometers, and other precision technologies. The objective was to evaluate the use of machine learning algorithms for the identification of lameness conditions in dairy cattle. In this study, 310 multiparous Holstein dairy cows from a herd in Northern Colorado were affixed with a leg-based accelerometer (Icerobotics(R) Inc, Edinburg, Scotland) to obtain the lying time (min/d), daily steps count (n/d), and daily change (n/d). Subsequently, study cows were monitored for 4 months and cows submitted for claw trimming (CT) were differentiated as receiving corrective claw trimming (CCT) or as being diagnosed with a lameness disorder and consequent therapeutic claw trimming (TCT) by a certified hoof trimmer. Cows not submitted to CT were considered healthy controls. A median filter was applied to smoothen the data by reducing inherent variability. Three different machine learning (ML) models were defined to fit each algorithm which included the conventional features (containing daily lying, daily steps, and daily change derived from the accelerometer), slope features (containing features extracted from each variable in Conventional feature), or all features (3 simple features and 3 slope features). Random forest (RF), Naive Bayes (NB), Logistic Regression (LR), and Time series (ROCKET) were used as ML predictive approaches. For the classification of cows requiring CCT and TCT, ROCKET classifier performed better with accuracy (> 90%), ROC-AUC (> 74%), and F1 score (> 0.61) as compared to other algorithms. Slope features derived in this study increased the efficiency of algorithms as the better-performing models included All features explored. However, further classification of diseases into infectious and non-infectious events was not effective because none of the algorithms presented satisfactory model accuracy parameters. For the classification of observed cow locomotion scores into severely lame and moderately lame conditions, the ROCKET classifier demonstrated satisfactory accuracy (> 0.85), ROC-AUC (> 0.68), and F1 scores (> 0.44). We conclude that ML models using accelerometer data are helpful in the identification of lameness in cows but need further research to increase the granularity and accuracy of classification.

animal behavior and cognition↗

In vitro antimicrobial activity of non-aureus Staphylococci and Mammaliicocci against Staphylococcus aureus and Streptococcus uberis and their relationship with the presence of intramammary infections in organic dairy cows

Prior studies have suggested that non-aureus staphylococci and mammaliicoci (NASM) possess inhibitory activity against mastitis pathogens. However, their impact on udder health outcomes and the mechanisms underlying this potential protective effect remain poorly understood. Our first objective was to identify NASM species on teat apices of organic dairy cows, assess their within-species phylogeny, and explore associations with presence of intramammary infections (IMI) and genomic features, including antimicrobial peptides (AMPs), virulence, and resistance genes. The second objective was to evaluate the in vitro antimicrobial activity of NASM isolates against mastitis pathogens and examine its associations with taxonomy, phylogeny, AMP genes, and IMI. Milk and teat apex swabs were collected weekly from 114 cows on two organic farms. Milk was cultured to identify Staphylococcus aureus (SAU) or Streptococcus spp. and Streptococcus-like organisms (SSLO) IMI. A case-control was designed to include cows with and without SAU or SSLO IMI. For each selected cow, the teat apex gauze swab collected during the week preceding IMI diagnosis (or corresponding time for controls) was aerobically cultured, and the taxonomy of isolates was determined using mass spectrometry. Isolates classified as NASM were subjected to whole genome sequencing using Illumina MiSeq. The inhibitory activity of NASM isolates was tested against SAU and Streptococcus uberis. Phylogenetic trees were constructed using Snippy and IQ-TREE. Genomes were assembled and annotated to identify species, AMP genes, virulence, and antimicrobial resistance markers. The in vitro antimicrobial activity of NASM varied across species and between cows with and without an IMI. Staphylococcus succinus was the species most frequently associated with highly inhibitory isolates, which were more prevalent in cows without IMI (19.4% vs 5.8%). Organic dairy cow teat apices harbored multiple NASM species and strains. All isolates had at least 1 AMP associated gene in their genome. In vitro antimicrobial activity was generally unrelated to clade membership, except for isolates classified as Staphylococcus succinus. Staphylococcus aureus had high virulence gene prevalence, while NASM species showed lower, species-specific prevalence. This study advances understanding of NASM antimicrobial activity and virulence potential.

microbiology↗

Exploring associations between the teat apex metagenome and Staphylococcus aureus intramammary infection risk in primiparous cows under organic directives

The primary objective of this study was to identify associations between teat apex microbiome and Staphylococcus aureus intramammary infection (IMI) risk in primiparous cows during the first 5 weeks after calving. We performed a case-control study using shotgun metagenomics of the teat apex and culture-based milk data collected longitudinally from 710 primiparous cows on 5 organic dairy farms. We observed a strong association between S. aureus DNA in the metagenomic teat apex data prior to parturition and the odds of S. aureus IMI after parturition (OR = 38.9, 95% CI: 14.84-102.21). Differential abundance analysis confirmed this association, with cases having a 23.8 higher log fold change (LFC) in abundance of S. aureus in their samples compared to controls. Of the most prevalent microorganisms in controls, those associated with a lower risk of post-calving S. aureus IMI included Microbacterium phage Min 1 (OR = 0.37, 95% CI: 0.25-0.53), Corynebacterium efficiens (OR = 0.53, 95% CI: 0.30-0.94), Kocuria polaris (OR = 0.54, 95% CI: 0.35-0.82), Micrococcus terreus (OR = 0.64, 95% CI: 0.44-0.93) and Dietzia alimentaria (OR = 0.45, 95% CI: 0.26-0.75). Microcin B17 was the most prevalent antibacterial peptide on the teat apex of cases and controls (99.7% in both groups). The predicted abundance of Microcin B17 was also higher in cases compared to controls (LFC 0.26). Cow and farm random effects often explained a large proportion of the observed variability in the teat apex microbiome, suggesting that our results need to be interpreted within the context of the random effects. IMPORTANCEIntramammary infections (IMI) caused by Staphylococcus aureus remain an important problem for the organic dairy industry. The microbiome on the external skin of the teat apex may play a role in mitigating S. aureus IMI risk, in particular the production of antimicrobial peptides (AMPs) by commensal microbes. However, current studies of the teat apex microbiome utilize a 16S approach, which precludes detection of genomics features such as AMPs. Therefore, further research using a shotgun metagenomic approach is needed to understand what role pre-partum teat apex microbiome dynamics play in IMI risk.

ecology↗