Basophils drive the resolution and promote wound healing in adult and aged mice
An active resolution is critical to control the duration of inflammation and limit its pathological consequences. Defects in resolution during wound healing allow the emergence of chronic wounds, a common complication in the elderly. Here, we show that basophils infiltrate the periphery of mouse skin wounds for at least three weeks, during both the inflammation and resolution phases of wound healing. Depletion of basophils induces an increased secretion of inflammatory molecules, accumulation and activation of pro-inflammatory leukocytes, and delays the wound healing response. Basophils particularly promote epidermal differentiation towards homeostasis in the wounds. Basophil-derived IL-4 and M-CSF drive partly their immunoregulatory and healing properties. Unexpectedly, aged mice basophils infiltrate more potently the wounds to promote inflammation resolution, showing a transcriptomic signature biased towards tissue remodeling. Thus, basophils are not pro-inflammatory but pro-resolution cells during an essential biological process such as skin wound healing. Unraveling basophil pro-resolution properties may reveal new strategies to fight chronic wounds in the elderly. SUMMARYThe resolution of inflammation is essential for homeostasis and becomes defective with age. Here we show basophil immunoregulatory properties promote resolution during skin wound healing, in both adult and aged mice.