Biology subjects

Pedigo, B. D.

Publications and source records attributed to Pedigo, B. D..

6 recordsLinked to original sources

bioRxiv2026

Cell-type-specific parallel pathways in the canonical cortical microcircuit

Information processing in the cortex depends on the integration of bottom-up and top-down signals through recurrent microcircuits spanning layers. Although the canonical microcircuit provides a framework for this integration, how these interactions are implemented at synapse resolution remains unclear. Here, we use large-volume electron microscopy reconstructions of mouse primary visual cortex to map the intralaminar and interlaminar connectivity of intratelencephalic (IT) neurons in layers 2/3 and 5. We find that layer 2/3 IT neurons formed a depth-dependent gradient of recurrent connectivity, with superficial (L2) and deeper (L3) neurons potentially forming two channels associated with top-down and bottom-up processing, respectively. These channels are preserved across layers via cell-type-specific pathways involving distinct L5 IT types, rather than collapsing into a single integrative pool. Moreover, each channel is regulated by a largely separate cohort of inhibitory interneurons, stabilizing recurrent excitation while limiting crosstalk. Together, these results reveal parallel, cell-type-specific processing streams embedded within the canonical circuit.

neuroscience↗

bioRxiv2026

A quantitative census of millions of postsynaptic structures in a large electron microscopy volume of mouse visual cortex

Neurons display remarkable sub-cellular specificity in their synaptic targeting, which varies by cell type--for example, excitatory neurons prefer to target the spines of other excitatory cells. Modern electron microscopy connectomes enable the study of this sub-cellular specificity and its context in a circuit at unprecedented scale and resolution. However, this scale has also made it challenging to create accurate and efficient methods for classifying and segmenting fine cell components (including spines) across entire volumes. Here, we present a cost-efficient computational pipeline for classifying postsynaptic targets and segmenting structures such as spines. Our method relies only on having a mesh representation of a neuron and avoids processing image or segmentation data directly. Instead, we leverage tools from geometry processing to create features capturing the local geometry of a neurons surface. We couple this core technique with computational and storage optimizations, enabling reliable deployment over hundreds of thousands of neurons for a few hundred dollars in cloud compute cost. We then show that a simple classifier trained on the MICrONS mouse visual cortex dataset can use these mesh-based features to accurately classify synapses as targeting somas, dendritic shafts, or spines (weighted F1 score 0.961). Using this pipeline, we create a map of the postsynaptic structures at over 207.3 million synapses in MICrONS. We present an overview of this census of postsynaptic targeting, finding expected patterns (e.g., excitatory neurons preferentially targeting excitatory spines) as well as unexpected exceptions (e.g., Layer 5 near-projecting and Layer 6 corticothalamic cells often connecting to excitatory neuron shafts). We also demonstrate that these tools can be used to detect spines receiving multiple synaptic inputs, revealing surprising variability in their frequency across cells even within a cell type. We make our postsynaptic target predictions available for study, as well as the code for the computational pipeline and cloud deployment. Beyond MICrONS, we find that the model generalizes well to the H01 connectome without retraining (weighted F1 score 0.949), indicating that these tools will be useful in future connectomics reconstructions. More generally, our work demonstrates that representations derived from neuronal meshes can be a scalable and generalizable primitive for describing morphologies.

neuroscience↗

bioRxiv2022

The connectome of an insect brain

Brains contain networks of interconnected neurons, so knowing the network architecture is essential for understanding brain function. We therefore mapped the synaptic-resolution connectome of an insect brain (Drosophila larva) with rich behavior, including learning, value-computation, and action-selection, comprising 3,013 neurons and 544,000 synapses. We characterized neuron-types, hubs, feedforward and feedback pathways, and cross-hemisphere and brain-nerve cord interactions. We found pervasive multisensory and interhemispheric integration, highly recurrent architecture, abundant feedback from descending neurons, and multiple novel circuit motifs. The brains most recurrent circuits comprised the input and output neurons of the learning center. Some structural features, including multilayer shortcuts and nested recurrent loops, resembled powerful machine learning architectures. The identified brain architecture provides a basis for future experimental and theoretical studies of neural circuits. One-Sentence SummaryWe generated a synaptic-resolution brain connectome and characterized its connection types, neuron types, and circuit motifs.

neuroscience↗

bioRxiv2022

Generative network modeling reveals quantitative definitions of bilateral symmetry exhibited by a whole insect brain connectome

Comparing connectomes can help explain how neural connectivity is related to genetics, disease, development, learning, and behavior. However, making statistical inferences about the significance and nature of differences between two networks is an open problem, and such analysis has not been extensively applied to nanoscale connectomes. Here, we investigate this problem via a case study on the bilateral symmetry of a larval Drosophila brain connectome. We translate notions of "bilateral symmetry" to generative models of the network structure of the left and right hemispheres, allowing us to test and refine our understanding of symmetry. We find significant differences in connection probabilities both across the entire left and right networks and between specific cell types. By rescaling connection probabilities or removing certain edges based on weight, we also present adjusted definitions of bilateral symmetry exhibited by this connectome. This work shows how statistical inferences from networks can inform the study of connectomes, facilitating future comparisons of neural structures.

neuroscience↗

bioRxiv2022

Bisected graph matching improves automated pairing of bilaterally homologous neurons from connectomes

Graph matching algorithms attempt to find the best correspondence between the nodes of two networks. These techniques have been used to match individual neurons in nanoscale connectomes - in particular, to find pairings of neurons across hemispheres. However, since graph matching techniques deal with two isolated networks, they have only utilized the ipsilateral (same hemisphere) subgraphs when performing the matching. Here, we present a modification to a state-of-the-art graph matching algorithm which allows it to solve what we call the bisected graph matching problem. This modification allows us to leverage the connections between the brain hemispheres when predicting neuron pairs. Via simulations and experiments on real connectome datasets, we show that this approach improves matching accuracy when sufficient edge correlation is present between the contralateral (between hemisphere) subgraphs. We also show how matching accuracy can be further improved by combining our approach with previously proposed extensions to graph matching, which utilize edge types and previously known neuron pairings. We expect that our proposed method will improve future endeavors to accurately match neurons across hemispheres in connectomes, and be useful in other applications where the bisected graph matching problem arises.

neuroscience↗

bioRxiv2019

Connectal Coding: Discovering the Structures Linking Cognitive Phenotypes to Individual Histories

Cognitive phenotypes characterize our memories, beliefs, skills, and preferences, and arise from our ancestral, developmental, and experiential histories. These histories are written into our brain structure through the building and modification of various brain circuits. Connectal coding, by way of analogy with neural coding, is the art, study, and practice of identifying the network structures that link cognitive phenomena to individual histories. We propose a formal statistical framework for connectal coding and demonstrate its utility in several applications spanning experimental modalities and phylogeny.

neuroscience↗