Biology subjects

Orain, N.

Publications and source records attributed to Orain, N..

3 recordsLinked to original sources

bioRxiv2026

Sarrella massiliensis gen. nov. sp. nov., a new gut genus isolated from the stool of a healthy donor

Strain Marseille-QA0792 (=CSUR QA0792, CECT 31199) was isolated from the feces of a healthy person using the culturomics method. The fecal sample originated from leftover material collected for a fecal microbiota transplantation (FMT) procedure, obtained during a routine screening in accordance with the guidelines of the French National Agency for Medicines and Health Products Safety (ANSM). This strain is Gram-stain-negative, rod-shaped, non-motile, and non-spore-forming. It is strictly anaerobic, with optimal growth at 37{degrees}C. Strain Marseille-QA0792 presented its highest 16S rRNA identity, 96.2%, with ''Merdibacter massiliensis'' strain Marseille-P3254 (not validly published) (accession number LT598590) and ANI similarity of 75.0% with the same species. Among validly published species, strain Marseille-QA0792 showed its highest 16S rRNA gene sequence similarity (91.5%) with Faecalicoccus acidiformans strain 37-2a (accession number HQ452864), whereas all computed average nucleotide identity (ANI) values were below 75%. Genome sequencing revealed a genome size of 2.67 Mb and a G+C content of 50.01%. This species is classified within the Erysipelotrichaceae family in the Bacillota phylum. Thus, we isolated for the first time a novel genus named Sarrella massiliensis gen. nov., sp. nov., strain Marseille-QA0792. This discovery highlights the importance of continuing culturomics efforts to explore the human microbiome.

microbiology↗

bioRxiv2022

Evidence of antibodies against SARS-CoV-2 in wild mustelids from Brittany (France)

In the French region of Brittany, mainly in the department of the Cotes dArmor, during the first semester of 2021, seropositivity for SARS-CoV-2 was detected in five wild mustelids out of 32 animals tested. Anti-SARS-CoV-2 IgG against at least four out of five recombinant viral proteins (S1 receptor binding domain, nucleocapsid, S1 subunit, S2 subunit and spike) were detected using automated western blot technique in three martens (Martes martes) and two badgers (Meles meles). An ELISA test also objectified seropositivities. Although the 171 qPCRs carried out on samples from the 33 mustelids were all negative, these preliminary results (observational study) nevertheless bear witness to infections of unknown origin. The epidemiological surveillance of Covid-19 in wildlife must continue, in particular with the tools of efficient serology.

microbiology↗

bioRxiv2020

Stool serology: development of a non-invasive immunological method for the detection of Enterovirus-specific antibodies in Congo gorilla feces

The incidence of poliovirus has significantly reduced by as much as 99.9% globally. Alongside this, however, vaccine-associated paralytic poliomyelitis has emerged. Recently, a new recombinant virus (Enterovirus C/Poliovirus) was identified in humans working as eco-guards and in gorillas in Democratic Republic of Congo, including one gorilla with polio-like sequelae. A strain of this recombinant virus (Ibou002) was also isolated from gorilla faeces. In order to assess the potential role of poliovirus infection, we have developed and optimised a protocol, based on the lyophilisation and solubilisation of small volumes of stool extracts, to detect specific antibodies. First, total immunoglobulins was detected in the concentrated stool extracts. Specific antibodies were then detected in 4/16 gorilla samples and 2/3 human samples by western blot using both the polio vaccine antigen and the Ibou002 antigen and by ELISA using the polio vaccine antigen. Humoral responses were greater with the Ibou002 antigen. We therefore suggest that this recombinant virus could lead to a polio-like disease in the endangered western lowland gorilla. The development of a non-invasive method to detect microorganism-specific immunoglobulins from faecal samples opens up new perspectives for the exploration of humoral responses of pathogens in animals and a greater understanding of zoonotic infectious diseases.

microbiology↗