Search bioRxiv⌕ Search

Biology subjects

Olt, J.

Publications and source records attributed to Olt, J..

2 recordsLinked to original sources

Novel algorithms deriving clinical performance measures from smartphone sensor data collected under a walking test

The modern ubiquity of smartphones which incorporate sensors such as inertial measurement units (IMU) and global positioning system (GPS) receivers, raises the possibility that they might be used as inexpensive devices for clinical performance testing. Here, we report on the development and testing of an app and associated statistical signal processing algorithms, to measure the basic gait properties of step rate, walking speed and distance under an outside short walk test experimental protocol, from digital accelerometry and GPS. We provide extensive details on the assumptions underlying the development of the algorithms. A small set of experiments across a range of conditions and individuals, demonstrate the reliability of the combined app, protocol and algorithms. With reasonable adherence to the test protocol, these experiments show 2% error across all metrics. The emphasis in this paper is on illustrating the design principles which all such systems, aiming to re-purpose consumer smartphones as clinical gait measurement devices, must address.

bioengineering↗

Spontaneous and coordinated Ca2+ activity of cochlear sensory and non-sensory cells drives the maturation of OHC afferent innervation

Outer hair cells (OHCs) are highly specialized sensory cells conferring the fine tuning and high sensitivity of the mammalian cochlea to acoustic stimuli. Here, by genetically manipulating spontaneous Ca2+ signalling in vivo, through a period of early postnatal development, we find that the refinement of OHC afferent innervation is regulated by complementary spontaneous Ca2+ signals originating in OHCs and non-sensory cells. OHCs fire spontaneous Ca2+ spikes during a narrow period of immature development. Simultaneously, waves of Ca2+ activity in the non-sensory greater epithelial ridge act, via ATP-induced activation of P2X receptors, to synchronize OHC firing, resulting in the refinement of their afferent innervation. In the absence of connexin channels Ca2+ waves are impaired, leading to a reduction in the number of ribbon synapses and afferent fibres on OHCs. We propose that the correct maturation of the afferent connectivity in OHCs requires experience-independent Ca2+ signals from sensory and non-sensory cells.

neuroscience↗