Anaerobic microbiota facilitate P. aeruginosa access to airway epithelia in a novel co-culture model of colonization
Strict and facultative anaerobic bacteria are widely associated with both acute and chronic airway disease. However, their potential role(s) in disease pathophysiology remain poorly understood due to inherent limitations of existing models and conflicting oxygen demands between anaerobes and host cells. To overcome these limitations, we optimized a dual oxic-anoxic culture (DOAC) platform that maintains an oxygen-limited microenvironment at the epithelial interface while host cells are oxygenated basolaterally. This approach enables host-bacterial co-culture for [~]24h, and here we demonstrate its utility via two applications. First, we show that anaerobe challenge results in epithelial mucus degradation, inflammatory marker gene expression, and enhanced pathogen colonization. Second, we combine DOAC with single cell RNA sequencing (scRNAseq) to reveal a cell type-specific transcriptional response of the airway epithelium to anaerobe infection. Together, these data illustrate the versatility of DOAC while revealing new insights into anaerobe-host interactions and their mechanistic contributions to airway disease pathophysiology.