Biology subjects

Nikanfar, M.

Publications and source records attributed to Nikanfar, M..

2 recordsLinked to original sources

bioRxiv2025

Growth inhibitory effect of cerebrospinal fluid of multiple sclerosis patients on HFF2 cells

Multiple sclerosis (MS) is an autoimmune disease characterized by demyelination of the central nervous system (CNS). Occurrence of immunogenic cell death (ICD) in this disease and expression of ICD markers in the CSF of MS patients are documented. However, cytotoxic effect of MS patients cerebrospinal fluid (CSF) on the cells is not reported. To evaluate the cytotoxic impact of CSF from MS patients on cell viability, human foreskin fibroblast (HFF2) cells were treated with CSF samples from individuals with MS and healthy controls. The results inferred that patient-derived CSF significantly declined the survival of HFF2 cells in comparison to the CSF of healthy individuals. This observation implies a cytotoxic environment and characteristic of patients CSF, which could be due to the presence of cellular-damaging factors such as calreticulin (CRT) in the patients CSF. The cytotoxic environment of CSF might reflect ICD occurrence in CNS of the patients and also could explain the progressive essence of the disease.

neuroscience↗

bioRxiv2024

Annexin A1, Calreticulin and High Mobility Group Box 1 are elevated in Secondary Progressive Multiple Sclerosis: Does Immunogenic Cell Death Occur in Multiple Sclerosis?

Multiple sclerosis (MS) is a chronic neuroinflammatory diseases characterized by demyelination of the nerve fibers. Immunogenic cell death (ICD) is a process, during which damaged and stressed cells release danger-associated molecular patterns (DAMPs) activating immune responses. This study aimed to elucidate the induction of ICD in MS diseases. To achieve this goal, the level of DAMPs including Annexin A1, calreticulin and HMGB1 was measured in the cerebrospinal fluid (CSF) of a secondary progressive multiple sclerosis (SPMS) patient in comparison to control group. Results showed significant upregulation (more than two- fold) of Annexin A1, calreticulin and HMGB1 in the CSF of the patient. Although further studies are suggested in this regard, this data could imply induction of ICD in MS. The proposed ICD might trigger immune response against neural cells resulting in neuroinflammation and demyelination in CNS in MS. Our observation could suggest inclusion of ICD interfering treatments in routine MS therapy.

immunology↗