Biology subjects

Nagalla, S.

Publications and source records attributed to Nagalla, S..

3 recordsLinked to original sources

bioRxiv2026

Rewiring systemic nodulation control to engineer heat-tolerant nitrogen fixation in Medicago truncatula and chickpea

Root nodule symbiosis allows legumes to meet their nitrogen requirements. However, the causes of symbiosis failure at high temperatures remain unknown. We demonstrated that elevated temperatures affect both the host and the microbial partner in Medicago truncatula and chickpea. Rhizobial viability and nod gene expression persisted under heat stress, but plant transcriptome analysis revealed a strong downregulation of the nodule organogenesis machinery. Nodule organogenesis was restored by constitutive overexpression of NIN-LBD16-NFYA1-NFYB16, miR2111, and CLE5 knockdown but failed to restore nitrogen fixation. In contrast, the genetic manipulation of the competence pathway involving CEP peptides significantly improved both nodulation and nitrogen fixation. Constitutive loss of the autoregulation of nodulation (AON) pathway in the SUNN alleles also rescued root nodulation and nitrogen fixation. Therefore, heat stress perturbs the balance between AON and the competence pathway, and genetic manipulation of these pathways is a promising approach to engineer thermotolerant nitrogen fixation in crop legumes.

plant biology↗

bioRxiv2025

LysM Receptor Proteins are Required for Ectomycorrhizal Symbiosis in Poplar

Successful symbiotic associations often rely on the perception of specific microbial signals by host plants through pattern recognition receptors. LysM receptor-like kinases are key receptors in both rhizobial and arbuscular mycorrhizal symbioses. In this study, we provide the first evidence that such LysM receptor-like kinases also control the establishment of ectomycorrhizal associations in poplar. Using CRISPR/Cas9-mediated genome editing, we knocked out three NFP-like candidate genes in various combinations. We examined their roles in the perception of the ectomycorrhizal fungus Laccaria bicolor, as well as chitin oligomers and lipo-chitooligosaccharides. We demonstrate that all three receptors are necessary for the recognition of symbiosis-related signals, calcium spiking, symbiotic gene expression, and ectomycorrhizal root phenotypes.

plant biology↗

bioRxiv2025

EPP1 is an ancestral component of the plant Common SymbiosisPathway

The success of plants on land has been enabled by mutualistic intracellular associations with microbes for 450 million years (Delaux and Schornack 2021). Because of their intracellular nature, the establishment of these interactions requires tight regulation by the host plants. In particular, three genes - SYMRK, CCaMK and CYCLOPS - form the core of an ancestral common symbiosis pathway (CSP) for intracellular symbioses, and are conserved since the most recent common ancestor of land plants (Radhakrishnan et al. 2020; Delaux et al. 2015; Wang et al. 2010; Parniske 2008). Here, we describe EPP1 as a fourth gene committed to the CSP. Among land plants, EPP1 is conserved only in species able to associate with at least one type of intracellular symbiont. We found that loss-of-function epp1 mutants or EPP1 knock-down lines in four clades of land plants - legumes, Solanaceae, monocots and bryophytes - are all impaired in their ability to associate with arbuscular mycorrhizal fungi. We discovered that the plasma membrane-localized receptor-like SYMRK phosphorylates EPP1 on a conserved serine residue and that this phosphorylation is essential for symbiosis. Using a gain-of-function approach, we demonstrate that EPP1 is upstream of the nuclear kinase CCaMK. We propose that EPP1 is an ancestral component of the essential pathway that has regulated plant symbiosis for half a billion years.

plant biology↗