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Mutterer, J.

Publications and source records attributed to Mutterer, J..

8 recordsLinked to original sources

The INO80-EEN complex prevents genomic rearrangements at protein coding genes regions

Plants are continuously exposed to a myriad of DNA-damaging agents, including environmental cues such as sunlight. At the cellular level, plants respond to DNA damage by activating DNA damage response (DDR) pathways, in which chromatin remodelers play an important role. Among them, the evolutionary conserved INO80 complex (INO80c) has been shown in Arabidopsis to play a key role in DDR, notably by positively regulating Homologous Recombination (HR). Arabidopsis EIN6 ENHANCER (EEN) is the homolog of Yeast INO EIGHTY SUBUNIT 6 and interacts with the N-terminal region of INO80 in the INO80c. Using plant phenotyping, cellular and molecular biology, and third-generation sequencing technology we investigated how INO80 and EEN regulate plant development and genome integrity. We uncovered new roles for INO80 and EEN in plant growth and for INO80 in fine tuning endoreduplication. In addition, linear genome analysis revealed an important and unexpected function for the INO80-EEN complex in preventing Protein Coding Genes (PCGs) from structural rearrangements in somatic tissue and upon exposure to UV-B. Therefore, our results shed new light on the previously overlooked roles of INO80 and EEN in protecting genome integrity at PCGs.

plant biology↗

Root hair growth phases are coordinated by cytoskeleton, nucleus dynamics and cell mechanics in Arabidopsis

Polar cell growth is a fundamental process across organisms, yet its coordination with nuclear movement and cytoskeleton dynamics remains underexplored. Focusing on Arabidopsis thaliana root hairs, we investigate these processes using high-resolution live imaging within microfluidics-based experiments. By incorporating data on cytoskeletal dynamics, nuclear positioning, and tip growth into a mathematical model, we analyse how their interactions shape the different growth phases that we reveal for the first time in this study. Chemical treatments and mutant analyses further support our model, revealing that timely cytoskeletal changes drive transitions between these growth phases, and correlate with shifts in nuclear movement and morphology. This regulation suggests a microtubule-actin crosstalk in the root hair subapical region. Additionally, we present novel findings on vacuole movement and cell stiffness, further refining our understanding of tip growth dynamics. Collectively, our work provides a comprehensive framework for understanding how transitions between growth phases are orchestrated in plant tip-growing cells.

cell biology↗

dsRNA-induced immunity targets plasmodesmata and is suppressed by viral movement proteins

Emerging evidence indicates that in addition to the well-recognized antiviral RNA silencing, dsRNA elicits responses of pattern-triggered immunity (PTI), likely contributing plant resistance against virus infections. However, compared to bacterial and fungal elicitor-mediated PTI, the mode-of-action and signaling pathway of dsRNA-induced defense remain poorly characterized. Here, using multi-color in vivo imaging by GFP mobility, staining of callose and plasmodesmal marker lines, we show that dsRNA-induced PTI restricts the progression of virus infection by triggering callose deposition at plasmodesmata, thereby likely limiting the macromolecular transport through these cell-to-cell communication channels. The plasma membrane-resident kinase module of SERK1 and BIK1/PBL1, plasmodesmata-localized proteins PDLP1/2/3 and calmodulin-like CML41, and Ca2+ signals are involved in the dsRNA-induced signaling leading to callose deposition at plasmodesmata and antiviral defense. In addition, unlike classical bacterial elicitor flagellin, dsRNA does not trigger detectable reactive oxygen species (ROS) burst, further substantiating a partially shared immune signaling framework with distinct features triggered by different microbial patterns. Likely as a counteract strategy, viral movement proteins from different viruses suppress the dsRNA-induced host response leading to callose deposition to achieve infection. Thus, our data support the new model of how plant immune signaling constrains the virus movement by inducing callose deposition at plasmodesmata and how viruses counteract this layer of immunity. One-sentence summarydsRNA-induced antiviral PTI targets plasmodesmata for callose deposition and is suppressed by virus-encoded movement proteins. IN A NUTSHELLO_ST_ABSBackgroundC_ST_ABSPlants use different defense mechanisms pathogens. The major mechanism that plants use for defense against viruses is known as RNA silencing. This mechanism is triggered by the presence of viral double-stranded (ds)RNA and uses small RNAs to inhibit viral replication by targeting the viral genome for degradation. Recently, it was found that dsRNA elicits antiviral defense also through a protein-mediated mechanism known as pattern-triggered immunity (PTI). However, the underlying mechanism of antiviral PTI and how viruses overcome this plant defense mechanism to cause infection is unknown. QuestionIn this study we asked how dsRNA-induced PTI acts to inhibit virus infection and whether we can identify components of the PTI signaling pathway. Moreover, we wanted to know how viruses overcome this plant host defense response in order to cause infection. FindingsWe demonstrate that dsRNA-induced PTI targets plasmodesmata (PD), the intercellular communication conduits in plant cell walls that viruses use to spread infection from cell to cell. By inducing the deposition of callose, dsRNA-induced PTI reduces PD permeability, thus restricting virus movement. We identified PTI signaling components required for dsRNA-induced PD callose deposition and delineate a PTI pathway showing important difference to PTI pathways triggered by microbial elicitors. Moreover, viral movement proteins (MPs) suppress the dsRNA-induced callose deposition response at PD. This leads to a new model of how plant immune signaling constrains virus movement and how viruses counteract this layer of immunity. Next stepsThis study calls upon the identification of the PTI dsRNA receptor and the mechanisms of PTI signaling (involving identified components such as SERK1, BIK1, calcium channels, CML41, PDLP1/2/3) and PTI suppression by MPs, and how dsRNA-induced PTI and RNA silencing are controlled during the spread of infection.

plant biology↗

Suppression of a dsRNA-induced plant immunity pathway by viral movement protein

The virome of plants is dominated by RNA viruses 1 and several of these cause devastating diseases in cultivated plants leading to global crop losses 2. To infect plants, RNA viruses engage in complex interactions with compatible plant hosts. In cells at the spreading infection front, RNA viruses replicate their genome through double-stranded RNA (dsRNA) intermediates and interact with cellular transport processes to achieve cell-to-cell movement of replicated genome copies through cell wall channels called plasmodesmata (PD) 3. In order to propagate, viruses also must overcome host defense responses. In addition to triggering the antiviral RNA silencing response, RNA virus infection also elicits pattern-triggered immunity (PTI) 4 whereby dsRNA, a hallmark of virus replication, acts as an important elicitor 5. This innate antiviral immune response is also triggered when dsRNA is applied externally and does not require sequence homology to the virus 5. However, the mechanism by which PTI restricts virus infection is not known. Here, we show that dsRNA inhibits the progression of virus infection by triggering callose deposition at plasmodesmata and the inhibition of transport through these cell-to-cell communication channels. The dsRNA-induced signaling pathway leading to callose deposition is independent of ROS production and thus distinguished from pathways triggered by bacterial and fungal elicitors. The dsRNA-induced host response at plasmodesmata is suppressed by the Tobacco mosaic virus movement protein (MP). Thus, the virus uses MP to inhibit innate dsRNA-induced immunity at plasmodesmata, which could be a general strategy of phytoviruses to overcome plant defenses and spread infection.

plant biology↗

A NYN domain protein directly interacts with DCP1 and is required for phyllotactic pattern in Arabidopsis.

In eukaryotes, general mRNA decay requires the decapping complex. The activity of this complex depends on its catalytic subunit, DCP2 and its interaction with decapping enhancers, including its main partner DCP1. Here, we report that in Arabidopsis, DCP1 also interacts with a NYN domain endoribonuclease, hence named DCP1-ASSOCIATED NYN ENDORIBONUCLEASE 1 (DNE1). Interestingly, we find DNE1 predominantly associated with DCP1 but not with DCP2 and reciprocally, suggesting the existence of two distinct protein complexes. We also show that the catalytic residues of DNE1 are required to repress the expression of mRNAs in planta upon transient expression. The overexpression of DNE1 in transgenic lines leads to growth defects and transcriptomic changes related to the one observed upon inactivation of the decapping complex. Finally, the combination of dne1 and dcp2 mutations, revealed a functional redundancy between DNE1 and DCP2 in controlling phyllotactic pattern formation in Arabidopsis. Our work identifies DNE1, a hitherto unknown DCP1 protein partner highly conserved in the plant kingdom and identifies its importance for developmental robustness. One-sentence summaryDNE1, a NYN domain protein interacts with the decapping activator DCP1 and, together with DCP2, specify phyllotactic patterns in Arabidopsis.

plant biology↗

Unravelling the puzzle of anthranoids metabolism in living plant cells using spectral imaging coupled to mass spectrometry

Vismione H (VH) is a fluorescent prenylated anthranoid produced by plants from the Hypericaceae family, with antiprotozoal activities against malaria and leishmaniosis. Little is known about its biosynthesis and metabolism in plants or its mode of action against parasites. When VH is isolated from Psorospermum glaberrimum, it is rapidly converted into madagascine anthrone and anthraquinone, which are characterized by markedly different fluorescent properties. To locate the fluorescence of VH in living plant cells and discriminate it from that of the other metabolites, an original strategy combining spectral imaging (SImaging), confocal microscopy and non-targeted metabolomics using mass spectrometry, was developed. Besides VH, structurally related molecules including madagascine, emodin, quinizarin as well as lapachol and fraxetin were analyzed. This strategy readily allowed a spatiotemporal characterization and discrimination of spectral fingerprints from anthranoids-derived metabolites and related complexes with cations and proteins. In addition, our study validates the capability of plant cells to metabolize VH into madagascine anthrone, anthraquinones and unexpected metabolites, leading to new hypotheses on the metabolism of anthranoids in plants.

plant biology↗

A quantitative gibberellin signalling biosensor reveals a role for gibberellins in internode specification at the shoot apical meristem

Growth at the shoot apical meristem (SAM) is essential for shoot architecture construction. The phytohormones gibberellins (GA) play a pivotal role in coordinating plant growth, but their role in the SAM remains mostly unknown. Here, we developed a ratiometric GA signalling biosensor by engineering one of the DELLA proteins, to suppress its master regulatory function in GA transcriptional responses while preserving its degradation upon GA sensing. We demonstrate that this novel degradation-based biosensor accurately reports on cellular changes in GA levels and perception during development. We used this biosensor to map GA signalling activity in the SAM. We show that high GA signalling is found primarily in cells located between organ primordia that are the precursors of internodes. By gain- and loss-of-function approaches, we further demonstrate that GAs regulate cell division plane orientation to establish the typical cellular organisation of internodes, thus contributing to internode specification in the SAM.

plant biology↗

Photodamage repair pathways contribute to the accurate maintenance of the DNA methylome landscape upon UV exposure

Plants are exposed to the damaging effect of sunlight that induces DNA photolesions. In order to maintain genome integrity, specific DNA repair pathways are mobilized. Upon removal of UV-induced DNA lesions, the accurate re-establishment of epigenome landscape is expected to be a prominent step of these DNA repair pathways. However, it remains poorly documented whether DNA methylation is accurately maintained at photodamaged sites and how photodamage repair pathways contribute to the maintenance of genome/methylome integrities. Using genome wide approaches, we report that UV-C irradiation leads to asymmetric DNA methylation changes. We identified that the specific DNA repair pathways involved in the repair of UV-induced DNA lesions, Direct Repair (DR) and Global Genome Repair (GGR), prevent the excessive alterations of DNA methylation landscape. Moreover, we identified that UV-C irradiation induced chromocenter reorganization and that photodamage repair factors control this dynamics. The methylome changes rely on misregulation of maintenance, de novo and active DNA demethylation pathways highlighting that molecular processes related to genome and methylome integrities are closely interconnected. Importantly, we identified that photolesions are sources of DNA methylation changes in both, constitutive and facultative heterochromatin. This study unveils that DNA repair factors, together with small RNA, act to accurately maintain both genome and methylome integrities at photodamaged silent genomic regions, strengthening the idea that plants have evolved sophisticated interplays between DNA methylation dynamics and DNA repair.

plant biology↗