Muthuvijayan, V. | Search bioRxiv

bioRxiv2024

Model-based fed-batch cultivation of Viola odorata plant cells exhibiting antimalarial and anticancer activity

Viola odorata is used in the indigenous medicine to treat respiratory tract disorders and is limited in availability. Bioprocess principles can be applied to develop sustainable methods to produce high-quality V. odorata biomass. To this effect, a modified stirred tank reactor and a balloon-type bubble column reactor were used to improve biomass production. Nutrient feeding strategies were developed using first principle-based mathematical modelling to achieve higher cell density in the reactor. Experimental validation of the fed-batch model-predicted strategy resulted in a two-fold enhancement in biomass production (32.2 g DW L-1) at the bioreactor level. Also, bioreactor-cultivated biomass extracts were tested for in vitro hemolytic, cytotoxic, anti-inflammatory, and in vivo antiplasmodial activities. This is the first report on fed-batch cultivation in bioreactors and the antiplasmodial activity of V. odorata. Overall, the bioactive potential of the in vitro-generated biomass extracts is found to be similar to that in the natural plant biomass extracts. HighlightsO_LICultivation of V. odorata cell suspension culture using a modified stirred tank and balloon-type bubble column bioreactors. C_LIO_LIBatch kinetic models were developed and extrapolated into a fed-batch model. C_LIO_LIEnhanced biomass production (32.2 g DW L-1) in bioreactors using a nutrient-feeding strategy. C_LIO_LIExtracts showed anti-inflammatory effects and up to 80 % inhibition of parasite growth, with no hemolytic activity. C_LIO_LIConfirmed antiplasmodial activity in vivo, effective alongside artesunate. C_LIO_LIIn vitro-generated biomass extracts showed comparable bioactive potential to that of natural plants. C_LI Graphical Abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=78 SRC="FIGDIR/small/618783v1_ufig1.gif" ALT="Figure 1"> View larger version (31K): org.highwire.dtl.DTLVardef@171c39aorg.highwire.dtl.DTLVardef@1e0eda5org.highwire.dtl.DTLVardef@121eddorg.highwire.dtl.DTLVardef@1a86215_HPS_FORMAT_FIGEXP M_FIG C_FIG

bioengineering↗

bioRxiv2022

Quaternary Ammonium Salt-Modified Isabgol (Psyllium) Scaffold as an Antibacterial Dressing for Improved Wound Healing

Chronic wounds require suitable treatment and management strategies for proper healing. Among other causes, infection delays the healing of wounds and increases the risk of wound-related complications. Healing of chronic wounds requires an ingenious biomaterial that is biocompatible and anti-infective to achieve effective wound management. In this study, a wound dressing with inherent antibacterial and biocompatible properties was developed to assist the healing process. Natural polysaccharide Isabgol was chemically modified with Epoxy propyl trimethyl ammonium chloride to render antibacterial activity to the material. This is the first report of such chemical modification of this polymer for biomedical applications. The modified material was freeze-dried to obtain scaffolds. 13C NMR and FTIR analysis confirmed the modification of the Isabgol polymer chains with EPTMAC. The scaffold exhibits an organized porous structure that allows the exchange of gases and nutrients through the matrix, as confirmed by SEM analysis. The material possesses excellent swelling properties up to 17 times its initial weight that allows it to absorb wound exudates and maintain a moist environment at the wound site. The scaffold is biodegradable, and thermally and mechanically stable. The material is anti-infective and can prevent infections at the wound site, which is one of the major causes of delayed wound healing. The developed scaffolds have been proven to be biocompatible and suitable for use in blood contact applications. Finally, since Isabgol is a low-cost raw material, the quaternary ammonium-modified Isabgol scaffold can be an affordable wound dressing material.

bioengineering↗