Biology subjects

Muller, H.-A. J.

Publications and source records attributed to Muller, H.-A. J..

2 recordsLinked to original sources

bioRxiv2018

SSPIM: a beam shaping toolbox for structured selective plane illumination microscopy

An important aim of the development of selective plane illumination microscopy (SPIM) is to present a completely open and flexible microscope set-up for nonspecialist users. Here, we report Structured SPIM (SSPIM), which provides an open-source, user-friendly and compact toolbox for beam shaping that can generate digital patterns for a wide range of illumination beams. SSPIM represents a toolbox to produce static, spherical Gaussian, Bessel and Airy beams by simple control of a Spatial Light Modulator (SLM). In addition, it is able to produce patterns for incoherent and coherent (lattice beam) array beam formation and tiling for all types of beams supported. We demonstrate the workflow and experimental and simulation results using the SSPIM toolbox. In final, the capability of the SSPIM is investigated with 3D imaging of Drosophila embryo using three different illumination beams such as scanned/dithered Gaussian, Bessel and Lattice beam which engineered with SSPIM. SSPIM toolbox is easy to use and applicable for a wide range of applications to generate and optimize the desired beam pattern and thus can help developing adaptation of the Open SPIM system towards a wider range of biological samples.

bioinformatics↗

bioRxiv2017

Requirement of the Dynein-adaptor Spindly for mitotic and post-mitotic functions in Drosophila

Spindly is a mitotic checkpoint protein originally identified as a specific regulator of Dynein activity at the kinetochore. In metaphase, Spindly recruits the Dynein/Dynactin complex, promoting the establishment of stable kinetochore-microtubule interactions and progression into anaphase. While details of Spindly function in mitosis have been worked out in cultured human cells and in the C. elegans zygote, the function of Spindly within the context of an organism has not yet been addressed. Here we present loss- and gain-of-function studies of Spindly in Drosophila. We investigated the requirements of distinct protein domains for the localisation and function of Spindly. We find that knock-down of Spindly results in a range of mitotic defects in the female germ line and during cleavage divisions in embryogenesis. Overexpression of Spindly in the female germ line is embryonic lethal and results in altered egg morphology. To determine whether Spindly plays a role in post-mitotic cells we altered Spindly protein levels in migrating cells and found that ovarian border cell migration is sensitive to the levels of Spindly protein. Our study uncovers novel functions of the mitotic checkpoint protein Spindly in Drosophila.

cell biology↗