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Mulec, I. M.

Publications and source records attributed to Mulec, I. M..

2 recordsLinked to original sources

Bacillus velezensis SQR9 sways the rhizosphere community and its sociality toward cooperation and plant growth promotion

According to the Lotka-Volterra competition model, intraspecific competition in plant-associated microbial communities should be stronger than interspecific competition. However, there is limited information on whether microbial communities follow this pattern and how disturbance by a newcomer affects them. Given the increasing popularity of probiotics, filling this knowledge gap could help guide future coexistence research. Here, we show that inoculation with a known probiotic, B. velezensis SQR9 shifts species co-occurrence patterns by decreasing the diversity of more distant species and promoting the growth of more closely related species, especially within the Bacillus community. By testing the sociality of Bacillus rhizosphere isolates, we then demonstrated that SQR9 increases the frequency of cooperative interactions in the Bacillus community, which may contribute to the promotion of plant growth. Finally, we provide an ecosystem framework comprising the strains genetic relatedness, metabolic niche space and social compatibility for the efficient and reliable assembly of Bacillus consortia. These findings shed new light on the ecological mechanisms underlying the beneficial effects of probiotics on host fitness.

microbiology↗

Housekeeping gene gyrA, a potential molecular marker for Bacillus ecology study

Bacillus is a ubiquitous microorganism and is one of the most commercially important species widely used in industry, agriculture and healthcare. Bacillus is relatively well understood at the single-cell level; however, molecular tools that determine diversity and ecology of Bacillus community are limited, which limits our understanding of how the Bacillus community works. In the present study, we investigated the potential of the housekeeping gene gyrA as a molecular marker for determining the diversity of Bacillus species. The amplification efficiency for Bacillus species diversity could be greatly improved by primer design. Therefore, we designed a novel primer pair gyrA3 that can detect at least 92 Bacillus species and related species. For Bacillus amyloliquefaciens, Bacillus pumilus, and Bacillus megaterium, we observed that the high variability of the gyrA gene allows for more detailed clustering at the subspecies level that cannot be achieved by the 16S rRNA gene. Since gyrA provides better phylogenetic resolution than 16S rRNA and informs on the diversity of the Bacillus community, we propose that gyrA gene may have broad application prospects in the study of Bacillus ecology.

microbiology↗