Biology subjects

Mosquera Vasquez, T.

Publications and source records attributed to Mosquera Vasquez, T..

3 recordsLinked to original sources

bioRxiv2025

Exploring the genetic factors of nitrogen use efficiency in potato

Nitrogen is an essential nutrient for plants, used by farmers to increase the yield of the crops. However, this practice increases greenhouse gases, negatively affecting the environment. Nitrogen Use Efficiency (NUE) is a trait that is beginning to be studied in some model species and in cereals due to its complex and novel trait nature. In potatoes, the information is scarcer. The study of NUE at the genetic level, based on a diverse population in potato materials, will contribute to the understanding of the genetic architecture of the trait. This research evaluated NUE in a Solanum tuberosum diploid potato genetic diversity panel from the Phureja group. The characterization of the trait was carried out in substrate conditions, for low and high levels of nitrogen for both the vegetative and the reproductive phase. Eighteen variables associated with NUE were measured, nine under low nitrogen and nine under high nitrogen conditions. A GWAS was conducted, and a total of 21 QTNs were identified as strongly associated with 11 phenotypic variables related to NUE in potato, along with a repertoire of 750 candidate genes associated with the trait. This research aimed to establish the basis for understanding the genetic architecture of NUE in Solanum tuberosum. Likewise, the knowledge derived is expected to be useful for plant breeding programs that begin to improve this trait.

genetics↗

bioRxiv2021

JASMONIC ACID PRIMING OF POTATO USES HYPERSENSITIVE RESPONSE-DEPENDENT DEFENSE AND DELAYS NECROTROPHIC PHASE CHANGE AGAINST PHYTOPHTHORA INFESTANS

One of the most important diseases affecting potato is late blight, caused by the oomycete Phtytophthora infestans. The use of jasmonic acid has been reported to reduce the progression of the disease in potato, but the defense mechanisms involved in this response are unknown. In this study we described the effect of jasmonic acid as a priming agent over time in the defense response of potato against the invasion of P. infestans. We observed that the initial stimulus generated by the exogenous application of jasmonic acid had an effect on the stomatal conductance of the treated tissue and activated StMYC2 expression. Results reveal a priming effect in plants inoculated 11 days after treatment with jasmonic acid, evidenced by an increased transcriptional induction of defense-associated genes, decrease in the number of necrotic lesions and an evident reduction of lesion area (72.23%). Furthermore, in this study, we show that the tested concentration of jasmonic acid does not have an adverse effect at the physiological level in plants, since variation in stomatal conductance was transient, no change in chlorophyll a fluorescence and no early senescence in leaves was observed.

plant biology↗

bioRxiv2019

NOVEL ORGAN-SPECIFIC GENETIC FACTORS FOR QUANTITATIVE RESISTANCE TO LATE BLIGHT IN POTATO

Potato, Solanum tuberosum, is one of the highest consumed food in the world, being the basis of the diet of millions of people. The main limiting and destructive disease of potato is late blight, caused by Phytophtora infestans. Here, we present a multi-environmental analysis of the response to P. infestans using an association panel of 150 accessions of S. tuberosum Group Phureja, evaluated in two localities in Colombia. Disease resistance data were merged with a genotyping matrix of 83,862 SNPs obtained by 2b-restriction site-associated DNA and Genotyping by sequencing approaches into a Genome-wide association study. We are reporting 16 organ-specific QTL conferring resistance to late blight. These QTL explain from 13.7% to 50.9% of the phenotypic variance. Six and ten QTL were detected for resistance response in leaves and stem, respectively. In silico analysis revealed 15 candidate genes for resistance to late blight. Four of them have no functional genome annotation, while eleven candidate genes code for diverse proteins, including one leucine-rich repeat kinase.

genetics↗