Biology subjects

Molloy, K.

Publications and source records attributed to Molloy, K..

2 recordsLinked to original sources

bioRxiv2024

The Molecular Architecture of the Nuclear Basket

The nuclear pore complex (NPC) is the sole mediator of nucle-ocytoplasmic transport. Despite great advances in understanding its conserved core architecture, the peripheral regions can exhibit considerable variation within and between species. One such structure is the cage-like nuclear basket. Despite its crucial roles in mRNA surveillance and chromatin organization, an architectural understanding has remained elusive. Using in-cell cryo-electron tomography and subtomogram analysis, we explored the NPCs structural variations and the nuclear basket across fungi (yeast; S. cerevisiae), mammals (mouse; M. musculus), and protozoa (T. gondii). Using integrative structural modeling, we computed a model of the basket in yeast and mammals that revealed how a hub of Nups in the nuclear ring binds to basket-forming Mlp/Tpr proteins: the coiled-coil domains of Mlp/Tpr form the struts of the basket, while their unstructured termini constitute the basket distal densities, which potentially serve as a docking site for mRNA preprocessing before nucleocytoplasmic transport

biophysics↗

bioRxiv2017

Auditory Figure-Ground Segregation is Impaired by High Visual Load

Figure-ground segregation is fundamental to listening in complex acoustic environments. An ongoing debate pertains to whether segregation requires attention or is automatic and pre-attentive. In this magnetoencephalography (MEG) study we tested a prediction derived from Load Theory of attention1 that segregation requires attention, but can benefit from the automatic allocation of any leftover capacity under low load. Complex auditory scenes were modelled with Stochastic Figure Ground stimuli (SFG2) which occasionally contained repeated frequency component figures. Naive human subjects passively listened to these signals while performing a visual attention task of either low or high load. Whilst clear figure-related neural responses were observed under conditions of low load, high visual load essentially abolished the neural response to the figure in auditory cortex (Planum Temporale, Heschls gyrus). We conclude that fundamental figure-ground segregation in hearing is not automatic but draws on shared resources across vision and audition.

neuroscience↗