Biology subjects

Merrihew, G.

Publications and source records attributed to Merrihew, G..

4 recordsLinked to original sources

bioRxiv2026

Spatiotemporal Mapping of Phosphorylation and Oxidation in the Pig Lens

BackgroundThe developmental pattern of the crystalline lens provides a unique model to study biological aging and its effects on the posttranslational modification of long-lived proteins. The orderly differentiation of lens fiber cells leads to a spatiotemporal gradient where mature, organelle-free fiber cells are packed in the lens nucleus surrounded by con-centric rings of successively younger fiber cells in the cortex. MethodsPig lenses were separated into six layers by dissolution in a hypotonic buffer. The changes in protein abundance, oxidation, and phosphorylation that occur across the spatiotemporal gradient of the lens were assessed quantitatively by using data independent acquisition label-free proteomic analysis of these six fractions. ResultsExpected changes in protein abundance of major lens protein which reflect the maturation process of lens fiber cells across the spatiotemporal gradient were found. Significant differences were noted in phosphorylation sites on crystallins, phakinin, and actin. Significant changes in oxidation of residues on essential lens proteins, as well as several glycolytic enzymes, were found across the spatiotemporal gradient. ConclusionDissolution of the lens followed by high resolution data-independent acquisition proteomics is a powerful technique for spatial mapping of protein abundance and posttranslational modification changes in the lens. The oxidation and phosphorylation sites noted in this study may play important roles in both lens development and cataractogenesis.

Molecular Biology↗

bioRxiv2020

Late-life restoration of mitochondrial function reverses cardiac dysfunction in old mice

Diastolic dysfunction is a prominent feature of cardiac aging in both mice and humans. We show here that 8-week treatment of old mice with the mitochondrial targeted peptide SS-31 (elamipretide) can substantially reverse this deficit. SS-31 normalized the increase in proton leak and reduced mitochondrial ROS in cardiomyocytes from old mice, accompanied by reduced protein oxidation and a shift towards a more reduced protein thiol redox state in old hearts. Improved diastolic function was concordant with increased phosphorylation of cMyBP-C Ser282 but was independent of titin isoform shift. Late-life viral expression of mitochondrial-targeted catalase (mCAT) produced similar functional benefits in old mice and SS-31 did not improve cardiac function of old mCAT mice, implicating normalizing mitochondrial oxidative stress as an overlapping mechanism. These results demonstrate that pre-existing cardiac aging phenotypes can be reversed by targeting mitochondrial dysfunction and implicate mitochondrial energetics and redox signaling as therapeutic targets for cardiac aging.

physiology↗

bioRxiv2019

A simple mass-action model predicts protein timecourses from mRNA trajectories during a dynamic response in two strains of S. cerevisiae

Although mRNA is a necessary precursor to protein, several studies have argued that the relationship between mRNA and protein levels is often weak. This claim undermines the functional relevance of conclusions based on quantitative analyses of mRNA levels, which are ubiquitous in modern biology from the single gene to the whole genome scale. Furthermore, if post-translational processes vary between strains and species, then comparative studies based on mRNA alone would miss an important driver of diversity. However, gene expression is dynamic, and most studies examining relationship between mRNA and protein levels at the genome scale have analyzed single timepoints. We measure yeast gene expression after pheromone exposure and show that, for most genes, protein timecourses can be predicted from mRNA timecourses through a simple, gene-specific, generative model. By comparing model parameters and predictions between strains, we find that while mRNA variation often leads to protein differences, evolution also manipulates protein-specific processes to amplify or buffer transcriptional regulation.

genomics↗

bioRxiv2018

Quantification of nuclear protein dynamics reveals chromatin remodeling during acute protein degradation

Sequencing-based technologies cannot measure post-transcriptional dynamics of the nuclear proteome, but unbiased mass-spectrometry measurements of chromatin-associated proteins remain difficult. In this work, we have combined facile nuclear sub-fractionation approaches with data-independent acquisition mass spectrometry to improve detection and quantification of nuclear proteins in human cells and tissues. Nuclei are isolated and subjected to a series of extraction conditions that enrich for nucleoplasm, euchromatin, heterochromatin and nuclear-membrane associated proteins. Using this approach, we can measure peptides from over 70% of the expressed nuclear proteome. As we are physically separating chromatin compartments prior to analysis, proteins can be assigned into functional chromatin environments to illuminate systems-wide nuclear protein dynamics. The integrity of nuclear sub-compartments were validated with immunofluorescence, which confirms the presence of key markers during chromatin extraction. We then apply this method to study the nuclear proteome-wide response to pharmacological degradation of the BET bromodomain proteins. BET degradation leads to widespread changes in chromatin composition, and we discover global HDAC1/2-mediated remodeling of chromatin previously bound by BET bromodomains. In summary, we have developed a technology for reproducible, comprehensive characterization of the nuclear proteome to observe the systems-wide nuclear protein dynamics.

systems biology↗