bioRxiv2025
The value of microbial natural product pathways extends beyond the chemicals they produce, as the enzymes they encode can be harnessed as biocatalysts. Microbial type II polyketide synthases (PKSs) are particularly noteworthy, as these enzyme assemblies produce complex polyaromatic pharmacophores. Combinatorial biosynthesis with type II PKSs has been described as a promising route for accessing never-before-seen bioactive molecules, but this potential is stymied in part by the lack of functionally compatible non-cognate proteins across type II PKS systems. Acyl carrier proteins (ACPs) are central to this challenge, as they shuttle reactive intermediates and malonyl building blocks between the other type II PKS domains during biosynthesis. Activating ACPs to their holo state via the phosphopantetheinyl transferase (PPTase)-catalyzed installation of a coenzyme A (CoA)-derived phosphopantetheine (Ppant) arm is critical to effectively study and strategically engineer type II PKSs, but not all ACPs can be activated using conventional PPTases. Here, we report the discovery of a previously unexplored non-actinobacterial PPTase from Dictyobacter vulcani sp. nov. W12 (vulcPPT). We explored its compatibility with both native and non-native ACPs, observing that vulcPPT activated all ACPs tested in this study, including a non-cognate, non-actinobacterial ACP which cannot be activated by the prototypical broad substrate PPTases AcpS and Sfp. Strategic optimization of phosphopantetheinylation reaction conditions increased apo to holo conversion. In addition to identifying a promising new promiscuous PPTase, this work establishes a road map for further investigation of PPTase compatibility and increases access to functional synthase components for use in combinatorial biosynthesis.