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Biology subjects

Martini, N.

Publications and source records attributed to Martini, N..

2 recordsLinked to original sources

Intercostal Space Prediction Using Deep Learning In Fully Endoscopic Mitral Valve Surgery

ObjectiveAbout 10 million people in Europe suffer from mitral valve incompetence. Majority of these entity is mitral valve prolapse in developed countries. Endoscopic mitral valve surgery is a relatively new procedure and preparation in the right intercostal space are crucial for success completion of the procedure. We aimed to explore clinical variables and chest X-rays in order to build most performant model that can predict the right intercostal space for thoracotomy.\n\nMethodsOverall 234 patients underwent fully endoscopic mitral valve surgery. All patients had preoperative two projection radiography. Intercostal space for right thoracotomy was decided by expert cardiac surgeons taking in consideration the height, weight, chest radiography, anatomical position of skin incision, nipple position and the sex. In order to predict the right intercostal space we have used clinical data and we have collected all radiographies and feed it to deep neural network algorithm. We have spitted the whole data-set into two subsets: training and testing data-sets. We have used clinical data and build an algorithm (Random Forest) in order to have reference model.\n\nResultsThe best-performing classifier was GoogLeNet neural network (now on we will reffera as Deep Learning) and had an AUC of 0.956. Algorithm based on clinical data (Random Forest) had AUC of 0.529 using only chest x-rays. The deep leaning algorithm predicted correctly in all cases the correct intercostal space on the training datasest except two ladies (96.08% ; with sensitivity of 97.06% and specificity 94.12 %, where the Random Forest was capable to predict right intercostal space in 60.78% cases with sensitivity of 93.33% and specificity 14.29 % (only clinical data).\n\nConclusionArtificial intelligence can be helpful to program the minimally invasive cardiac operation, for right intercostal space selection for thoracotomy, especially in non optimal thoraxes (example, obese short ladies). It learned from the standard imaging (thorax x-ray) which is easy, do routinely to every patient.

bioinformatics↗

Mud sticks after all: Chronically implanted microelectrodes but not electrical stimulation cause c-fos expression along their trajectory

The goal of CNS implanted devices is to build a stable brain-machine-interface. The brain tissue response to the foreign body limits the functionality and viability of this brain-machine connection. Notably the astrocytic glial scar formation and inflammation with resulting neuronal cell loss is considered to be responsible for the signal deterioration over time. We chronically implanted a polyimide microelectrode in the dorsolateral striatum of rats. First, we analyzed the c-fos immunoreactivity following high frequency stimulation (HFS) of the dorsolateral striatum and second, using GFAP and ED1 immunocytochemistry, the brain tissue response. Acute as well as chronic HFS showed no significant change of neuronal c-fos expression in the dorsolateral striatum and corresponding cortical areas. We found that the sole chronic implantation of a polyimide microelectrode leads to a reaction of the surrounding neurons, i.e. c-fos expression, along the implantation trajectory. We also observed the formation of a glial scar around the microelectrode with a low number of inflammation cells. Histological and statistical analysis of NeuN positive cells showed no kill zone, which accompanied neuronal cell death around the implantation site.

neuroscience↗