Martinez, C. M. | Search bioRxiv

bioRxiv2021

Galvanic current activates the NLRP3 inflammasome to promote type I collagen production in tendon

The NLRP3 inflammasome coordinates inflammation in response to different pathogen- and damage-associated molecular patterns, being implicated in different infectious, chronic inflammatory, metabolic and degenerative diseases. In chronic tendinopathies lesions, different non-resolving mechanisms produce a degenerative condition that impairs tissue healing, complicating their clinical management. Percutaneous needle electrolysis consist in the application of a galvanic current and is emerging as a novel treatment for tendinopathies. Here we found that galvanic current activates the NLRP3 inflammasome and the induction of an inflammatory response promoting a collagen-mediated regeneration of the tendon. This study establish the molecular mechanism of percutaneous electrolysis for the treatment of chronic lesions and the beneficial effects of an induced inflammasome-related response.

immunology↗

bioRxiv2020

Environmental Drivers of Variation in Labial Teeth Number in Ascaphus Tadpoles

Morphological variation is often maintained by complex and interrelated factors, complicating the identification of underlying drivers. Tadpole oral morphology is one such trait that may be driven by the independent and interacting effects of the environment and variation in developmental processes. Although many studies have investigated tadpole oral morphological diversity among species, few have sought to understand the drivers that underlie intraspecific variation. In this study, we investigated potential drivers of labial tooth number variation among populations of two species of tailed frogs, the Rocky Mountain tailed frog (Ascaphus montanus) and the Coastal tailed frog (A. truei). We counted labial teeth from 240 tadpoles collected across elevation (both species) and latitudinal (A. truei) gradients, providing a natural temperature gradient. We tested the effects of developmental stage and local temperature conditions on labial tooth number. We found that labial tooth number variation was independently affected by both developmental stage and local temperature, as well as the interacting effects of these two variables (pseudo-R2 = 67-77%). Our results also uncovered consistent patterns in labial tooth row formula across the ranges of both species; however, A. truei tadpoles from northern British Columbia had a unique bifurcation of a posterior tooth row. This study highlights the diversity in intraspecific tadpole oral morphology and the interacting processes that drive it.

ecology↗