Marsden, K. | Search bioRxiv

bioRxiv2025

Early disruptions in vitamin D receptor signaling induces persistent developmental behavior deficits in zebrafish larvae

A critical function of the nervous system is to rapidly process sensory information and initiate appropriate behavioral responses. Defects in sensory processing and behavior selection are commonly observed in neuro-psychiatric conditions including anxiety, autism (ASD), and schizophrenia. The etiology of sensory processing disorders remains equivocal; however, it is hypothesized that extrinsic environmental factors can play fundamental roles. In this study we examine the importance of vitamin D (1, 25-dihydroxyvitamin D3) receptor signaling during early life stage development on sensory processing and neurobehavioral health outcomes. While vitamin D has traditionally been associated with mineral ion homeostasis, accumulating evidence suggests non-calcemic roles for vitamin D including early neurodevelopment. Here we demonstrate that systemic disruption of vitamin D receptor (VDR) signaling with a conditional dominant negative (dnVDR) transgenic zebrafish line results in specific visual and acoustic sensorimotor behavior defects. Induction of dnVDR between 24-72 hours post fertilization (hpf) results in modulation of visual motor response with demonstrate attenuation in acute activity and hypolocomotion across multiple swimming metrics when assayed at 6- and 28-days post fertilization (dpf). Disruption in VDR signaling additionally resulted in a strong and specific attenuation of the Long-Latency C-bends (LLC) within the acoustic startle response at 6 dpf while Short-Latency C-bends (SLC) were moderately impacted. Pre-pulse inhibition (PPI) was not impacted in young larvae however exhibited a significantly attenuated response at 28 dpf suggesting an inability to properly modulate their startle responses later in development and persistent effects of VDR modulation during early development. Overall, our data demonstrate that modulation of vitamin D signaling during critical windows of development irreversibly disrupts the development of neuronal circuitry associated with sensory processing behaviors which may have significant implications to neurobehavioral health outcomes.

neuroscience↗

bioRxiv2022

The spatial distribution and temporal trends of livestock damages caused by wolves in Europe

Wolf populations are recovering and expanding across Europe, causing conflicts with livestock owners. To mitigate these conflicts and reduce livestock damages, authorities spend considerable resources to compensate damages, support damage prevention measures, and manage wolf populations. However, the effectiveness of these measures remains largely unknown, especially at larger geographic scales. Here we compiled incident-based livestock damage data across 21 countries for the years 2018, 2019 and 2020, during which 39,262 wolf-caused incidents were reported from 470 administrative regions. We found substantial regional variation in all aspects of the data, including the primary target species, the density of damages, their seasonal distribution, and their temporal trend. More than half of the variation in damage densities across regions is explained by the area of extensively cultivated habitats occupied by wolves and namely natural grasslands and broad-leaved forests. Regional variation in husbandry practices and damage prevention, while difficult to quantify at a continental scale, appear important factors to further modulate these incidents. As illustrated with detailed data from Germany, for instance, the relationship between the number of wolf units and damages is diminishing over time, suggesting some adaptation of livestock owners and local authorities to their presence, for example by increasing prevention efforts. As we argue, temporal trends of damage incidents, which are robust to variation in data collection across regions, are thus informative about the local intensity of the wolf-human conflict. We estimated increasing trends for the majority of regions, reflecting the current expansion of wolves across the continent. Nonetheless, many of these increases were moderate and for more than one third of all regions, trends were negative despite growing wolf populations, thus indicating that wolf-livestock conflicts can be successfully mitigated with proper management.

ecology↗