Biology subjects

Marin, R. H.

Publications and source records attributed to Marin, R. H..

3 recordsLinked to original sources

bioRxiv2024

Chronic Stress effects on Immune-Neuroendocrine matrix in Coturnix coturnix, analyses from an ontogenetic perspective

Immune-neuroendocrine characteristics can be used to classify individuals according to their physiological profiles or phenotypes (INPs). In avian models such as quail and domestic chickens, three subgroups based on INPs have been defined: Lewis-like (pro-inflammatory polarization), Fischer-like (anti-inflammatory polarization), and an intermediate INP. This study investigates the stability and alterations of INPs throughout ontogeny, from juvenile to adult stages in four time-points including an exposure to unpredictable and diverse chronic stress (CS) during early adulthood. We measured corticosterone levels, pro-(IFN-{gamma} and IL-1{beta}) and anti-inflammatory (IL-13, IL-4) cytokines, phytohemagglutinin (PHA-P) lymphoproliferative response, anti-sheep red blood cells antibody (Ab SRBC) response, and leukocyte distribution frequency. Cluster analyses were conducted to classify bird based on their similarities across all analyzed variables, to thereby establish their INP at each time point. The extreme Lewis- or Fischer-like profiles were less represented in juvenile and pre-stress adult birds showing a higher proportion of individuals with an intermediate profile. Following CS exposure, the prevalence of Lewis-like and Fischer-like profiles increased. This shift persisted 10 weeks later as birds matured to an advanced egg-laying stage, with females predominantly exhibiting the Fischer-like INP, and males the Lewis-like INP. The observed shift in INP distribution following CS towards more polarized Lewis- and Fisher-like profiles implies a more even representation of the three observed profiles and may reflect inter-individual differences in physiological response to CS associated to particular coping strategies. A more even INPs distribution could provide the population with a greater advantage when facing diverse environmental challenges. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=108 SRC="FIGDIR/small/577005v3_ufig1.gif" ALT="Figure 1"> View larger version (13K): org.highwire.dtl.DTLVardef@15b1dfdorg.highwire.dtl.DTLVardef@10df09dorg.highwire.dtl.DTLVardef@300532org.highwire.dtl.DTLVardef@a543e0_HPS_FORMAT_FIGEXP M_FIG C_FIG

physiology↗

bioRxiv2023

Thanks to repetition, dustbathing detection can be automated combining accelerometry and wavelet analysis

Dustbathing is performed by many groups of birds, including Galliformes. It consists of a well-defined orderly sequence of movements. Repetitive changes in body position during dustbathing can be automatically detected through data processing of body mounted accelerometer recordings, specifically the complex Morlet continuous wavelet transform. The approach was tested in 13 adult male Japanese quail (Coturnix japonica) fitted with a backpack containing a triaxial accelerometer and video-recorded during at least 6h. Rhythmicity (period 25-60s) in the y-axis acceleration vector is reflected as large power values, and is associated almost exclusively to dustbathing events. Thus, by implementing a threshold value we detected events automatically with an accuracy of 80% (range 66-100%). We show potential uses for characterizing temporal dynamics (e.g. daily rhythms) of dustbathing and for the assessment of intra- and inter-individual variability over long-term studies, even within large complex environments (e.g. natural environments or breeding facilities). Summary statementWe propose a method for automatically detecting dustbathing (i.e a behavior performed by many groups of birds, including Galliformes) from triaxial accerometer recoding using a wavelet technique.

animal behavior and cognition↗

bioRxiv2022

Context- and scale-dependent effects of thymol bioactivity on biological networks: contributions from quail under heat stress

A defining feature of healthy function is adaptability, the capacity to dynamically respond to novel and/or unpredictable factors, such as exposition to environmental challenges. Here we explore the role of context (i.e., order in which factors are applied) on the configuration of networks of behavioral and physiological traits from Japanese quail that were supplemented dietary thymol prior, jointly or after onset of a chronic heat stress protocol (i.e., supplementation strategies). Basal diet and standard environmental temperature were used as controls. We begin by showing that each supplementation strategy evolved differently over the 5-week experimental period in regard to body weight and feed- intake. At the end of this experimental period, context-dependency was also observed in the non- trivial functional relationships among 27 traits from 4 subsystems from distinct spatial-temporal scales. When considered separately, whole-organism level subsystems (i.e. somatic maintenance and egg production traits), are predominantly functionally related to environmental temperature. Conversely, at the molecular level, the live[r]s antioxidant system response is fundamentally dominated by the supplementation strategy. Interestingly, the serums antioxidant system shows an intermediate response, not dominated by a given factor. Overall, network configurations were highly dependent on context, and could be associated with specific induced physiological states. Our work constitutes the first study that includes network, integrative, and experimentally comparative analyses applied to the field of dietary supplementation under environmental challenges. This perspective could help understand complex biological responses important for developing efficient and welfare orientated supplementation protocols for farm animals and for interpreting the background in this field.

physiology↗