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Marchesi, F.

Publications and source records attributed to Marchesi, F..

2 recordsLinked to original sources

SIGHT: an immersive Virtual Reality platform for reinforced learning of optics and functional neuroanatomy of vision

Virtual reality (VR) offers immersive and interactive learning environments that can improve student engagement and 3D visualization. However, its application in medical education is mostly limited to clinical settings and its potential for better understanding complex concepts, or empathy with the patients, remains underexplored. Here, we describe SIGHT (Simulated Immersive Guidance for Human Training), an immersive VR application, designed to teach core concepts in the physics and functional neuroanatomy, or neurophysiology of human vision. Its two integrated learning modules allow first-person experience of normal and pathological conditions: the optics module enables users to manipulate lenses, experience refractive errors such as myopia, presbyopia, and astigmatism and correct them through appropriate lens selection; the neurophysiology module allows learners to navigate the visual pathways from the retina to the visual cortex and to simulate lesions, experiencing the corresponding visual field deficits. User authentication and interactive evaluation steps provide analytical feedback of the experience and learning process. A pilot group of medical students reported high usability, engagement and deeper understanding of the vision-related concepts, showing how the approach of SIGHT can support experiential learning in medical education.

scientific communication and education↗

Temporal differentiation of bovine airway epithelial cells grown at an air-liquid interface

The respiratory epithelium is exposed to assault by toxins and pathogens through the process of inhalation, which has numerous implications on both human and animal health. As such, there is a need to develop and characterise an in vitro model of the airway epithelium to study respiratory pathologies during infection or toxicology experiments. This has been achieved by growing airway epithelial cells at an air-liquid interface (ALI). Characterisation of ALI models are not well-defined for airway epithelial cells derived from non-human species. In this study we have fully characterised a bovine airway epithelial cell models (AECM) grown at an ALI in relation to ex vivo tissue. The morphology of the model was monitored at three day intervals, to identify the time-period at which the culture was optimally differentiated. The model was shown to be fully-differentiated by day 21 post-ALI. The culture formed a stereotypical pseudostratified, columnar epithelium containing the major cell types of the bronchial epithelium (ciliated-, goblet- and basal cells). Once fully differentiated the bovine AECM displayed both barrier function, through the formation of tight-junctions, and active mucociliary clearance, important properties of the mucosal barrier. The bovine bronchial epithelial cells remained stable for three weeks, with no evidence of deterioration or dedifferentiation. The window in which the model displayed full differentiation was determined to be between day 21-42 post-ALI. Through comparison with ex vivo tissue derived from donor animals, our bovine AECM was shown to be highly representative of the in vivo bovine bronchial epithelium and can be utilised in the study of respiratory pathologies.

microbiology↗