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Madduppa, H.

Publications and source records attributed to Madduppa, H..

2 recordsLinked to original sources
bioRxiv2020

Genetic population subdivision of Blue Swimming Crab (Portunus pelagicus) across Indonesia inferred from mitochondrial DNA: implication to sustainable fishery

The blue swimming crab (BSC), Portunus pelagicus (Linnaeus 1758), inhabits coastal areas of Southeast and East Asia, and is one of high fisheries commodity with export value for Indonesia and global market demand increasing annually. However, the data of genetic diversity and their spatial connectivity of populations in Indonesia are not yet known, which is important to inform unit stock management and sustainable fisheries. This study aimed to determine genetic diversity and differentiation of blue swimming crab across Indonesia populations under different Fishery Management Area, and their spatial genetic connectivity, as well as implications for sustainable fishery. A total of 297 individuals were amplified using cytochrome oxidase I mitochondrial DNA. This study shown highest value of haplotype and nucleotide diversity in the eastern part of Indonesia, where exploitation is relatively low. Significant genetic differentiation between populations (FST = 0.954; p < 0.001) and the fishery management regions (FST = 0.964; p < 0.001) were revealed. Low spatial connectivity was observed between populations in a distance of at least more than 60 kilometers. This study suggests that BSC populations in Indonesia likely have several unit stock, and preferably different fisheries management plan and action across the region thoroughly and simultaneously is effective for management and their sustainable conservation.

molecular biology↗
bioRxiv2020

Genetic connectivity and diversity of endangered species the scalloped hammerhead shark Sphyrna lewini (Griffith & Smith 1834) population in Indonesia and Western Indian Ocean

The scalloped hammerhead shark Sphyrna lewini is an endangered species which expected to population declined worldwide including in Indonesia due to overexploited. However, there is a lack of information regarding recent population structure to promote proper management and conservation status in Indonesia. This study aimed to investigate the genetic diversity, population structure and connectivity of S. lewini population in Indonesia from three major sharks landing sites in Aceh (n= 41), Balikpapan (n= 30), Lombok (n= 29), and additional sequences retrieved from West Papua (n= 14) and Western Indian Ocean population (n= 65). Analyses of mitochondrial CO1 gene successfully identified a total of 179 sequences of S. lewini with an average 594 bp nucleotide with 40 polymorphic loci in 4 haplotypes for Indonesian population and 8 haplotypes for Western Indian Ocean. The overall values of genetic diversity in Indonesia was high (Hd= 0.7171; {pi}= 0.0126), with the highest was in Aceh (Hd= 0.6683; {pi}= 0.0198), and the lowest was in Papua (Hd= 0.1429; {pi}= 0.0005), while in Western Indian Ocean the overall value was fairly low (Hd= 0.2322; {pi}= 0.0010). The AMOVA and FST revealed three significant population subdivisions in Indonesia (FST= 0.4415; p < 0.001) with separated population for Aceh and West Papua, and a mixing population between Balikpapan and Lombok (FST= 0.044; p = 0.089), whereas relatively no significant differentiation within population in Western Indian Ocean (FST= -0.0131; p = 0.6011), and significant different level showed by Indonesian population compared with Western Indian Ocean population (FST= 0.7403; p < 0.001). The construction of haplotype network exhibited evidence of gene flow and haplotype sharing between populations. This result indicated a complex and limited connectivity population of S. lewini in Indonesia, and between Western Indian Ocean in regional scale which need co-management action across region.

molecular biology↗