Biology subjects

Macchione, M.

Publications and source records attributed to Macchione, M..

2 recordsLinked to original sources

bioRxiv2019

Nanoscale topography templates the organization of stable clathrin/AP-2 structures

Membrane shape is a key feature of many cellular processes, including cell differentiation, division, migration, and trafficking. The development of nanostructured surfaces allowing for the in situ manipulation of membranes in living cells is crucial to understand these processes, but this requires complicated and limited-access technologies. Here, we investigate the self-organization of cellular membranes by using a customizable and bench top method allowing to engineer 1D SiO2 nanopillar arrays of defined sizes and shapes on high-performance glass compatible with advanced microscopies. As a result of this original combination, we provide a mapping of the morphology-induced modulation of the cell membrane mechanics, dynamics and steady-state organization of key protein complexes implicated in cellular trafficking and signal transduction.

biophysics↗

bioRxiv2019

TORC2 Affects Endocytosis through Plasma Membrane Tension

Target Of Rapamycin complex 2 (TORC2) is a conserved protein kinase that regulates multiple plasma membrane (PM)-related processes including endocytosis. Direct, chemical inhibition of TORC2 arrests endocytosis but with kinetics that are relatively slow and therefore inconsistent with signaling being mediated solely through simple phosphorylation cascades. Here, we show that, in addition to regulation of the phosphorylation of endocytic proteins, TORC2 also controls endocytosis by modulating PM tension. Elevated PM tension, upon TORC2 inhibition, impinges on endocytosis at two different levels: first, by severing the bonds between the PM adaptor proteins Sla2 and Ent1 and the actin cytoskeleton; and, second, by hindering recruitment of Rvs167, an N-BAR-containing protein important for vesicle fission, to endocytosis sites. These results underline the importance of biophysical cues in the regulation of cellular and molecular processes.

cell biology↗