Combining two Genetic Sexing Strains allows sorting of non-transgenic males for Aedes genetic control
Chemical control of disease vectoring mosquitoes Aedes albopictus and Aedes aegypti is costly, unsustainable, and increasingly ineffective due to the spread of insecticide resistance. The Sterile Insect Technique is a valuable alternative but is limited by the slow, error-prone, and wasteful sex-separation requirement. Here, we present four Genetic Sexing Strains (two for each Aedes species) based on fluorescence markers linked to the m and M sex loci, allowing for the isolation of transgenic males. Furthermore, we demonstrate how combining these sexing strains enables the production of non-transgenic males. Scaling-up would allow the sorting of 100,000 first instar male larvae in under 1.5 hours with an estimated 0.01-0.1% female contamination. Cost-efficiency analyses revealed that using these strains could result in important savings while setting up and running a mass-rearing facility. Altogether, these Genetic Sexing Strains should enable a major upscaling in control programmes against these important vectors. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=136 SRC="FIGDIR/small/483912v2_ufig1.gif" ALT="Figure 1"> View larger version (42K): org.highwire.dtl.DTLVardef@761545org.highwire.dtl.DTLVardef@6e80f2org.highwire.dtl.DTLVardef@d3911dorg.highwire.dtl.DTLVardef@1dc19ce_HPS_FORMAT_FIGEXP M_FIG C_FIG