Lightly, T. J. | Search bioRxiv

bioRxiv2026

Utility of Kirschner Wires as Prosthetic Implants to Investigate Establishment and Treatment of Biofilm Associated Infections in a Galleria mellonella Model

ObjectivesBacteriophage (phage) therapy is being explored as a strategy for clinical control of periprosthetic joint infections, addressing the challenges of antibiotic-resistant bacteria and limitations around the complete eradication of methicillin-resistant Staphylococcus aureus biofilms by conventional antibiotic treatment. Additionally, phage-antibiotic combinations may enhance treatment success through synergistic interactions. This study aimed to compare the efficacy of phage Silviavirus remus (Remus) and vancomycin, independently and together, against S. aureus biofilms on Kirschner-wire (K-wire) implants using Galleria mellonella as an in vivo model, and to assess the biocompatibility of K-wire implantation. ResultsSurvival of G. mellonella larvae implanted with S. aureus biofilms and treated with Remus ([~]3 x 104 PFU/worm) and/or vancomycin (5 {micro}g/worm) after 72 hours was assessed using Kaplan-Meier curves. Statistical analyses among treatment groups were performed using log-rank tests at p-value < 0.10. Implantation of uncolonized K-wires with treatment administration did not affect survival (100%). Compared with untreated biofilm-infected larvae (77%), vancomycin alone (97%; p = 0.023) or in combination with Remus (93%; p = 0.087) improved survival, whereas Remus alone did not increase survival (67%, p = 0.33). Scanning electron microscopy confirmed the presence of biofilm-associated attachment of S. aureus on K-wires, although adherence was non-uniform.

microbiology↗

bioRxiv2019

Phenylacetyl-CoA, not phenylacetic acid, mediates quorum sensing-regulated virulence in Burkholderia cenocepacia

During phenylalanine catabolism, phenylacetic acid (PAA) is converted to phenylacetyl-CoA (PAA-CoA) by a ligase, PaaK, and then epoxidized by a multicomponent monooxygenase, PaaABCDE, before further degradation to the TCA cycle. In the opportunistic pathogen Burkholderia cenocepacia, loss of paaABCDE attenuates virulence factor expression, which is under control of the LuxIR-like quorum sensing system, CepIR. To further investigate the link between CepIR-regulated virulence and PAA catabolism, we created knockout mutants of the first step of the pathway (PAA-CoA synthesis by PaaK) and characterized them in comparison to a paaABCDE mutant using liquid chromatography/mass spectrometry (LC-MS/MS) and virulence assays. We found that while loss of PaaABCDE decreased virulence, deletion of the paaK genes resulted in a more virulent phenotype than the wild type strain. Deletion of either paaK or paaABCDE led to higher levels of released PAA but no differences in internal accumulation, compared to wild type. While we found no evidence of direct cepIR downregulation by PAA-CoA or PAA, a low virulence cepR mutant reverted to a virulent phenotype upon removal of the paaK genes. On the other hand, removal of paaABCDE in the cepR mutant did not impact its attenuated phenotype. Together, our results suggest an indirect role for PAA-CoA in supressing B. cenocepacia CepIR-activated virulence.\n\nImportanceThe opportunistic pathogen Burkholderia cenocepacia uses a chemical signal process called quorum sensing (QS) to produce virulence factors. In B. cenocepacia, QS relies on the presence of the transcriptional regulator CepR, which upon binding QS signal molecules, activates virulence. In this work, we found that even in the absence of CepR, B. cenocepacia can elicit a pathogenic response if phenylacetyl-CoA, an intermediate of the phenylacetic acid degradation pathway, is not produced. Instead, accumulation of phenylacetyl-CoA appears to attenuate pathogenicity. Therefore, we have discovered that it is possible to trigger virulence in the absence of CepR, challenging the classical view of activation of virulence by this QS mechanism. Our work provides new insight into the relationship between metabolism and virulence in opportunistic bacteria. We propose that, in the event that QS signaling molecules cannot accumulate to trigger a pathogenic response, a metabolic signal can still activate virulence in B. cenocepacia.

microbiology↗