Langlais, C. | Search bioRxiv

bioRxiv2024

Going with the flow: leveraging reef-scale hydrodynamics for upscaling larval-based restoration

Anthropogenic pressures are impacting coastal marine ecosystems, necessitating large-scale interventions to accelerate recovery. Propagule-based restoration holds the potential for restoring shallow coastal systems at hectare scales by harnessing natural dispersal. However, predicting propagule dispersal remains challenging due to the complex hydrodynamic nature of coastal marine ecosystems and the complex behaviours of marine propagules. To improve predictions of fine-scale larval dispersal patterns, we developed a 3D reef-scale ([~]30 m resolution) dispersal model for Lizard Island, Australia, with the aim to predict the effect of island scale hydrodynamics on the distribution of coral spawn slicks and larvae. Using in situ field observations, and dispersal simulations, we assessed the models capability to (1) forecast hydrodynamic conditions, (2) predict coral spawn slick convergence zones for collection efforts, and (3) identify optimal locations and timeframes where high particle residence time may enhance local settlement following larval delivery to damaged reefs. Predictions of convergence zones in the upper water column aligned well with field observations of coral spawn slicks. At the reef benthos, the model captured variability in current speed and direction at [~]58% of studied locations. At other locations, the model did not resolve hydrodynamic conditions due to sheltering effects and associated hydrodynamic processes occurring at a scale below 50 m. At locations where the model performed well, propagules could remain within a one-hectare area around the delivery site for 5 to 15 hours depending on locations and the timing of larval release. These high retention conditions were infrequent but occurred at least once at 15 of the 25 studied sites. Observations of local currents a posteriori confirmed model predictions, showing periods of little water movement lasting from 6.5 to 15 hours. Overall, our study highlights fine-scale dispersal modelling as a key tool for scaling up larval-based reef restoration, while also acknowledging the need for better predictions of local conditions in complex, shallow environments. Applications of fine-scale modelling, coupled with local knowledge of reproductive timing and larval behavioural ecology, assist with the mass collection of propagules upon release and in identifying areas and times of optimal larval deployment to achieve the greatest impact.

ecology↗

bioRxiv2021

Characterisation of FADD interactome reveals novel insights into FADD recruitment and signalling at the Death Inducing Signalling Complex (DISC)

Fas-associated death domain protein (FADD) plays a vital role in the extrinsic apoptotic pathway, where it forms an essential component of the death-inducing signaling complex (DISC). However, the precise early molecular events that facilitate recruitment of FADD to the DISC remain poorly defined. Using affinity purification and mass spectrometry we investigated the FADD interactome in untreated cells and following death receptor stimulation to identify novel FADD-interacting proteins. As expected, in death receptor-stimulated samples our analysis identified key components of the DISC such as Caspase-8. In addition, we identified novel binding partners including Transferrin Receptor 1 (TfR1) and Myosin Light Chain Kinase 2 (MYLK2) that are able to modulate FADD recruitment to the DISC and consequently downstream apoptotic signaling. TfR1 is pre-associated with FADD and recruited into the DISC; moreover, our data reveal that TfR1 is also pre-associated with the death receptors, TRAIL-R1 and TRAIL-R2, thereby functioning as a key regulator of DISC formation. In the case of MYLK2, specific binding of FADD to MYLK2 in non-apoptotic cells sequesters FADD from other DISC components ensuring aberrant apoptosis is not initiated. Furthermore, MYLK2 enzymatic activity is required to for it to translocate, in complex with FADD, to sites of DISC-mediated death receptor oligimerization. Taken together, our study highlights the important role that additional novel FADD binding partners play in the regulation of death receptor-mediated apoptotic cell death, in part by modulating FADD recruitment to the DISC.

biochemistry↗