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Lage, A.

Publications and source records attributed to Lage, A..

2 recordsLinked to original sources
bioRxiv2023

Species composition determines bioplastics production in photosynthetic microbiomes: strategy to enrich cyanobacteria PHB-producers

The aim of this study was to set the operating mode in regards to nutrients, temperature and light to use as a strategy to enrich a microbiome rich in cyanobacteria in polyhidroxybutyrate (PHB)-producers in order to enhance this biopolymer production. Alternate growth and accumulation phases were conducted for 179 days in a 3 L photobioreactor. Although, presence of green microalgae potentially reduced PHB production, the microbiome produced up to 22 % dry cell weight (dcw) PHB. Results suggested that this methodology could be applied to a robust microbiome rich in cyanobacteria to boost PHB production.

bioengineering↗
bioRxiv2023

New strategy for bioplastic and exopolysaccharides production: Enrichment of field microbiomes with cyanobacteria

Seven photosynthethic microbiomes were collected from field environmental samples to test their potential in polyhydroxybutirate (PHB) and exopolysaccharides (EPS) production, two alternatives to chemical-based polymers. Microscope observations together with microbial sequence analysis revealed the microbiome enrichment in cyanobacteria after culture growth under phosphorus limitation. PHB and EPS production were studied under three culture factors (phototrophy, mixotrophy and heterotrophy) by evaluating and optimizing the effect of three parameters (organic and inorganic carbon and days under light:dark cycles) by Box-Behnken design. Results showed that optimal conditions for both biopolymers synthesis were microbiome-dependent; however, the addition of organic carbon boosted PHB production in all the tested microbiomes, producing up to 14%dcw PHB with the addition of 1.2 g acetate{middle dot}L-1 and seven days under light:dark photoperiods. The highest EPS production was 59 mg{middle dot}L-1 with the addition of 1.2 g acetate{middle dot}L-1 and four days under light:dark photoperiods. The methodology used in this article is suitable for enriching microbiomes in cyanobacteria, and for testing the best conditions for bioproducts synthesis for further scale up.

bioengineering↗