Biology subjects

Kuo, H.-Y.

Publications and source records attributed to Kuo, H.-Y..

2 recordsLinked to original sources

bioRxiv2019

Developmental Characterization of Zswim5 Expression in the Progenitor Domains and Tangential Migration Pathways of Cortical Interneurons in the Mouse Forebrain

GABAergic interneurons play an essential role in modulating cortical networks. The progenitor domains of cortical interneurons are localized in developing ventral forebrain, including the medial ganglionic eminence (MGE), caudal ganglionic eminence (CGE), preoptic area (POA) and preoptic hypothalamic border domain (POH). Here, we characterized the expression pattern of Zswim5, an MGE-enriched gene in the mouse forebrain. At E11.5 to E13.5, prominent Zswim5 expression was detected in the subventricular zone (SVZ) of MGE, CGE, POA and POH of ventral telencephalon in which progenitors of cortical interneurons resided. At E15.5 and E17.5, Zswim5 remained detectable in the SVZ of pallidal primordium (MGE). Zswim5 mRNA was markedly decreased after birth and was absent in the adult forebrain. Interestingly, Zswim5 expression pattern resembled the tangential migration pathways of cortical interneurons. Zswim5-positive cells in the MGE appeared to migrate from the MGE through the SVZ of LGE to overlying neocortex. Indeed, Zswim5 was co-localized with Nkx2.1 and Lhx6, markers of progenitos and migratory cortical interneurons. Double labeling showed that Mash1/Ascl1-positive cells did not express Zswim5. Zswim5 expressing cells showed none or at most low levels of Ki67 but co-expressed Tuj1 in the SVZ of MGE. These results suggest that Zswim5 is immediately upregulated as progenitors exiting cell cycle to become postmitotic. Given that recent studies have elucidated that the cell fate of cortical interneurons is determined shortly after postmitotic, the timing of Zswim5 expression in early postmitotic cortical interneurons suggests a potential role of Zswim5 in regulation of neurogenesis and tangential migration of cortical interneurons.

neuroscience↗

bioRxiv2019

A normative study of modified spatial context memory test in middle and older individuals

In view of the spatial context memory function of hippocampus complex region, we designed a modified spatial context memory test (SCMT) and try to early identify amnesic mild cognitive impairment.\n\nAll participants with non-dementia were recruited and divided 2 age groups (55-65 years and 66-75 years) and 3 education levels (6-9 years, 10-12 years and more than 12 years). The mini-mental state examination, visual associative memory test, visual construction retention test, and logical memory subtest of the Wechsler memory scale-III were used to evaluate the cognition state of the individuals. Spatial-context memory test version I with combination of a spatial memory paradigm and a real-life event included 3 subtests of navigation, scene-event association and people-object association.\n\nA total of 147 individuals were confirmed to be normal in cognition in the assessment of the neuropsychological test battery. Regardless of age or level of education, there was no significance in perseveration errors and the retrieval of learned-well navigation information. In the Scene-Event Association test, the subjects with a low level of education seemed to have relative difficulty to quickly classify new information and establish the effective cues to retrieval. The subjects with a high level of education, the performance of spatial-context memory were negative correlation to the age. In the People-Object association test, the subjects in all six groups made no perseveration errors, but older subjects required more time to retrieval, and this situation was more prominent in the subjects with high level education.\n\nWe establish a normal age- and education-adjusted SCMT score in the middle and elder individuals.

neuroscience↗