Biology subjects

Kunimasa, K.

Publications and source records attributed to Kunimasa, K..

2 recordsLinked to original sources

bioRxiv2026

An integrated scalable process for adherent cultivated meat production: From proliferative cell selection to safety-verified product development

Cultivated meat can contribute to the global food supply; therefore, establishing efficient production processes is an urgent task to meet the growing demand for sustainable protein. Although cell culture technology has historically focused on suspension cells in the pharmaceutical and, subsequently, cultivated meat industries, the development of efficient processes for most adhesion-dependent cells has lagged, with limited examples reported to date. Therefore, this study used primary duck liver-derived adherent cells to develop and evaluate an integrated three-step meat production process involving pre-culture to select highly proliferative cells, packed-bed bioreactor expansion for mass production, and final processing, including packaging and heating. The established process allowed the efficient growth of selected cell populations while maintaining their proliferative characteristics. Moreover, the developed product met the microbiological and heavy metal safety criteria. Comprehensive compositional analysis (nutritional, amino acid, and fatty acid profiles) revealed that the product exhibited a protein profile distinct from that of conventional duck liver paste, along with a unique lower-fat signature. As productivity is dependent on cell doubling time, culture duration and monthly production were estimated for cells from various animal species. Overall, this study established a practical integrated production process for cultivated meat using adherent cells, providing a technological foundation for cellular agriculture applicable to diverse cell types and useful for future food supply diversification. GraphicalAbstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=74 SRC="FIGDIR/small/698307v1_ufig1.gif" ALT="Figure 1"> View larger version (23K): org.highwire.dtl.DTLVardef@18b1148org.highwire.dtl.DTLVardef@11bbcecorg.highwire.dtl.DTLVardef@102d99org.highwire.dtl.DTLVardef@21af6f_HPS_FORMAT_FIGEXP M_FIG C_FIG

bioengineering↗

bioRxiv2024

Environmental Impacts of Serum-free Food-grade and Complex Culture Medium Production for Cultivated Meat

Culture medium accounts for most of the environmental impact of cultivated meat production. This study quantitatively evaluated and analyzed the environmental impact of producing a culture medium consisting of serum-free, food and complex ingredients for cultivated meat by performing a life cycle assessment (LCA) based on activity data at the laboratory scale. In addition, specific measures were proposed to reduce the environmental impact further. LCAs were performed at current and future production scales. This study also evaluated the impact of multiple electricity sources on the environmental impact of culture medium production. Energy, animal-derived materials, and expendables at the current scale, and energy, animal-derived materials, amino acids, and glucose at future scale are hotspots in the environmental impact of this culture medium production. The production of serum substitutes accounts for most of the environmental impact. As the scale shifts, the environmental impacts are expected to decrease by more than 70% in all impact categories. As the composition of electricity sources changed, the impact on certain categories decreased. However, as the share of renewable energy increased, the impact on land use also increased significantly. This study promotes the practical application of new culture-media for low-cost and low-environment-impact cultivated meat. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=108 SRC="FIGDIR/small/611339v2_ufig1.gif" ALT="Figure 1"> View larger version (52K): org.highwire.dtl.DTLVardef@ac6daorg.highwire.dtl.DTLVardef@1852ef4org.highwire.dtl.DTLVardef@cdbbe8org.highwire.dtl.DTLVardef@eb8703_HPS_FORMAT_FIGEXP M_FIG O_FLOATNOGraphical abstract.C_FLOATNO SYNOPSIS. This study evaluated the environmental impact of culture-medium production for cultivated meat and reports that energy, animal-derived materials, expendables, amino acids and glucose are hotspots of this impact. C_FIG

ecology↗