Biology subjects

Kumar, E.

Publications and source records attributed to Kumar, E..

3 recordsLinked to original sources

bioRxiv2023

Investigation of Biomedical Cell Image Cryptography Based on RC4 Technique

Enormous data permeates various facets of social life, including public administration, community services, and scientific research, owing to the advent of new technologies. With the escalation of data transportation concerns, its significance in our daily lives has magnified. Particularly in the medical field, preserving information integrity during data transit has become an urgent priority. For instance, safeguarding massive electronic medical files like MRI images from cyberattacks and data loss poses a formidable challenge. Encryption and decryption techniques are employed as a solution. While numerous image types have undergone testing using cryptography techniques, biomedical images have received limited attention. In this article, We implemented RC4, pixel shuffling, and steganography to encrypt and decrypt cell images. Evaluating the encryption and decryption performance of these cell images involved testing cell viability, live/dead ratio, and morphology.

bioengineering↗

bioRxiv2023

Investigation of Two-Component Hydrogel System for the Tissue Regeneration from Simulation

Tissue degenerative diseases pose significant global health challenges. Currently, hydrogel therapy stands as a promising approach to address these conditions. To explore its potential, we developed a computational simulation model to mimic hydrogel behavior accurately and precisely measure its degradation rate while incorporating dynamics associated with cell growth. This model aimed to investigate the relationship between a two-component hydrogel system and cell growth, assessing its feasibility for tissue regeneration. Our analysis revealed that the nutritional support for neural stem cells exceeds that of bone marrow cells, followed by other types of cells. This is significant considering the challenge in culturing neural stem cells compared to the relative ease of culturing other cell types. Additionally, we found that there isnt a single solution to determine the optimal conditions for cell growth. Different tissue regeneration processes require distinct conditions to establish what could be considered as suitable growth environments. Lastly, its important to note that these suitable conditions can be fine-tuned by adjusting various parameters.

biochemistry↗

bioRxiv2020

KDM5 inhibition offers a novel therapeutic strategy for the treatment of KMT2D mutant lymphomas

Loss-of-function mutations in KMT2D are a striking feature of the germinal centre (GC) lymphomas, resulting in decreased H3K4 methylation and altered gene expression. We hypothesised that inhibition of the KDM5 family, which demethylates H3K4me3/me2, would re-establish H3K4 methylation and restore the expression of genes repressed upon loss of KMT2D. KDM5-inhibition increased H3K4me3 levels and caused an anti-proliferative response in vitro, which was markedly greater in both endogenous and CRISPR-edited KMT2D mutant DLBCL cell lines, whilst tumour growth was inhibited in KMT2D mutant xenografts in vivo. KDM5-inhibition reactivated both KMT2D-dependent and -independent genes, resulting in diminished B-cell receptor signalling and altered expression of BCL2 family members, including BCL2 itself, allowing it to synergise with agents targeting these pathways. KDM5-inhibition may offer an effective therapeutic strategy for ameliorating KMT2D loss-of-function mutations in GC-lymphomas.Statement of significance We detail a novel way of reverting the effects of loss-of-function mutations in the histone methyltransferase KMT2D by inhibiting the KDM5 demethylase family, increasing levels of H3K4me3 and restoring expression of KMT2D regulated genes.Competing Interest StatementThe authors have declared no competing interest.View Full Text

cancer biology↗