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Kruyt, F. A.

Publications and source records attributed to Kruyt, F. A..

2 recordsLinked to original sources

The Unfolded Protein Response Sensor PERK Mediates Mechanical Stress-induced Maturation of Focal Adhesion Complexes in Glioblastoma Cells

Stiffening of the brain extracellular matrix (ECM) in glioblastoma leads to mechanical stress, which is known to contribute to tumor formation and progression. Previously, we found that protein kinase R (PKR)-like endoplasmic reticulum kinase (PERK), a component of the unfolded protein response (UPR), plays a role in the adaptation of glioblastoma stem cells (GSCs) to matrix stiffness through PERK/FLNA dependent F-Actin remodeling. Here, we found that increases in matrix stiffness induces differentiation of GSCs that was not seen in PERK deficient GSCs. Furthermore, we investigated whether PERK is involved in detecting changes in matrix stiffness through focal adhesion complex (FAC) formation and maturation, which are instrumental for transmitting ECM dependent signaling. In PERK deficient GSCs, Vinculin and Tensin expression was decreased, while Talin and Integrin-{beta}1 expression was kept at the same level compared to PERK proficient cells. In addition, in the absence of PERK, Tubulin expression is sharply increased coupled with low Vimentin expression, which was observed as an opposite trend in the presence of PERK. In conclusion, our study reveals a novel role for PERK in regulating the formation of FACs during matrix stiffening, possibly associated with its regulatory capacity in F-Actin remodeling.

cancer biology↗

TGF-beta promotes microtube formation in glioblastoma through Thrombospondin 1

Microtubes (MTs), cytoplasmic extensions of glioma cells, are important cell communication structures promoting invasion and treatment resistance through network formation. MTs are abundant in chemoresistant gliomas, in particular glioblastomas (GBMs), while they are uncommon in chemosensitive IDH-mutant and 1p/19q co-deleted oligodendrogliomas. To identify potential signaling pathways involved in MT formation we performed a bioinformatics analysis of TCGA data showing that the TGF-{beta} pathway is highly activated in GBMs compared to oligodendroglial tumors. In particular we observed that signaling pathways involved in extracellular matrix organization are differentially expressed between these tumor entities. Using patient-derived GBM stem cell lines, we demonstrated that TGF-{beta}1 stimulation promotes enhanced MT formation and communication via Calcium signaling. Inhibition of the TGF-{beta} pathway significantly reduced MT formation and its associated invasion in vitro and in vivo. Downstream of TGF-{beta}, we identified thrombospondin 1 (TSP1) as a potential mediator of MT formation in GBM through SMAD activation. TSP1 was upregulated upon TGF-{beta} stimulation and enhanced MT formation, which was inhibited by TSP1 shRNAs in vitro and in vivo. In conclusion, TGF-{beta} and its downstream mediator TSP1 are important mediators of the MT network in GBM and blocking this pathway could potentially help to break the complex MT driven invasion/ resistance network.

cancer biology↗