Biology subjects

Kluiszo, E.

Publications and source records attributed to Kluiszo, E..

3 recordsLinked to original sources

bioRxiv2026

Mesoscopic Fluorescence Imaging of Light-Triggered Chemotherapeutic Release in Cancer Spheroid Models

Peritoneal micrometastases (micromets) remain a major barrier to durable cytoreduction in ovarian and other intra-abdominal cancers, because lesions can be difficult to visualize and are often resistant to systemic therapy. Liposomal doxorubicin (Dox) improves pharmacokinetics but can be limited by slow intratumoral release. Porphyrin-phospholipid (PoP) liposomes enable near-infrared light-triggered release of Dox (chemophototherapy (CPT)), creating an opportunity for intraoperative, fluorescence-guided treatment planning and monitoring. Here, we evaluate a laparoscopic fluorescence imaging platform for quantifying light-triggered drug delivery in 2D monolayers and 3D spheroid cluster models. Dox fluorescence increased linearly with administered LC-Dox-PoP concentration in both SCC2095sc and SKOV-3 cultures (R2 = 0.97-0.98 in 2D; R2 = 0.98 in spheroid clusters over 1-9 {micro}g/mL). Laparoscope-derived fluorescence measurements agreed with standard well-plate reader measurements (R2 = 0.89-0.96). Porphyrin fluorescence provided stronger, complementary contrast for localizing spheroid constructs and decreased after activation light exposure, consistent with photobleaching during triggered release. Together, these results support a quantitative imaging framework for fluorescence-guided monitoring of light-triggered liposomal drug release, with potential to inform individualized CPT dosimetry for peritoneal micrometastases. These findings in SCC2095sc (oral squamous cell carcinoma) additionally suggest relevance of fluorescence-guided CPT for head and neck/oral cancer, where localized post-resection adjuvant treatment may improve control of residual disease.

bioengineering↗

bioRxiv2026

Depth-Sensitive Optical Property Characterization Using Multi-Frequency Laparoscopic SFDI

Accurate knowledge of tissue absorption (a) and reduced scattering [Formula] parameters are required to plan and monitor laparoscopic chemophototherapy (CPT) in ovarian cancer, including light dosimetry and quantitative fluorescence mapping of porphyrin-phospholipid (PoP) photobleaching and light-triggered doxorubicin (Dox) release. We implemented a depth-sensitive, multi-frequency laparoscopic spatial frequency domain imaging (SFDI) framework to improve optical-property estimation in layered tissue. A DMD-based laparoscope imaged two-layer phantoms with controlled optical contrasts and superficial thicknesses. Spatial-frequency subsets associated with different penetration depths were independently fit to recover a and [Formula], and compared with a two-layer diffusion model. Recovered [Formula] values remained bounded by the known layer references and shifted monotonically toward the superficial value as spatial frequency and top-layer thickness increased, approaching a single-layer response at high frequency/thick layers. Quantitative model comparison showed {delta}-P1 variants outperformed the standard diffusion approximation, reducing RMSPE between modeled and measured [Formula] to 0.8-6.5% (silicone/silicone) and 1.6-8.3% (silicone/intralipid), whereas SDA errors reached [~]13.8% and 21.1%, respectively. This approach demonstrates multi-frequency laparoscopic SFDI as a practical initial step for depth-sensitive fluorescence correction for individualized CPT treatment planning and monitoring.

bioengineering↗

bioRxiv2025

Quantitative Fluorescence Imaging of Porphyrin Phospholipid Photobleaching and Light Activated Liposomal Doxorubicin Release Using Wide-field and Laparoscopic SFDI in an Ovarian Cancer Model

Chemophototherapy (CPT) is an emerging cancer treatment that leverages the synergistic effects of photodynamic therapy (PDT) and chemotherapy. This approach utilizes photosensitizers like Porphyrin Phospholipid (PoP) and Doxorubicin (Dox) to enable phototriggered drug release and targeted tumor destruction. In this study, we present the development and validation of a wide-field laparoscopic spatial frequency domain imaging (SFDI) system, designed to improve intraoperative quantitative fluorescence imaging and monitoring of PoP photobleaching, a PDT-driven effect for tumor destruction, and light-activated Dox release, which facilitates targeted chemotherapeutic drug delivery in an ovarian cancer model. Compared to previous flexible endoscopic imaging methods, our laparoscopic SFDI system offers enhanced spatial coverage, enabling accurate wide-field optical property quantification in minimally invasive surgical settings. Using this system, we performed quantitative fluorescence imaging in vivo to obtain absolute concentrations of PoP and Dox fluorescence, correcting for tissue absorption and scattering effects. This capability allows for precise assessment of PoP photobleaching and Dox release kinetics with improved spatial resolution. Fluorescence imaging revealed a significant reduction in PoP concentration in tumor regions post-illumination, demonstrating the PDT-mediated photobleaching effect and successful light-triggered drug release activation for chemo-induced tumor destruction. The ability to differentiate PoP and Dox fluorescence in a laparoscopic system underscores its potential for real-time intraoperative monitoring of CPT efficacy. These findings establish wide-field laparoscopic SFDI as a promising tool for guiding minimally invasive photodynamic therapy and targeted drug delivery in clinical settings.

bioengineering↗