Biology subjects

Kheyfets, V. O.

Publications and source records attributed to Kheyfets, V. O..

3 recordsLinked to original sources

bioRxiv2024

On Monitoring Brain Health from the Depths of Sleep: Feature Engineering and Machine Learning Insights for Digital Biomarker Development

BackgrounSingle-channel sleep electroencephalography (EEG) is a promising technology for creating cost-effective and widely accessible digital biomarkers for monitoring brain health. Sleep, notable for its numerous connections to brain health, is of particular interest in this context. Indeed, several of the best studied and widely recognized risk factors for neurodegenerative disease are also connected to aspects of sleep physiology, including biological sex, hypertension, diabetes, obesity/metabolic dysregulation, and immune system dysfunction. In this study, we utilize the unique signal characteristics of slow wave sleep (SWS) oscillatory events as features in machine learning models to predict underlying biological processes that are highly relevant to brain health. Our objective is to establish a foundation for algorithms capable of effectively monitoring physiological processes in sleep that directly and indirectly inform brain health using single-channel sleep EEG as a functional metric of brain activity. MethodsUtilizing data from the Cleveland Family Study, we analyzed 726 overnight polysomnography recordings to extract features from slow waves and adjacent oscillatory events. Advanced signal processing and machine learning techniques, including random forest models, were employed to engineer features and predict health-related outcomes such as age, cerebrovascular risk factors, endocrine functions, immune system activity, and sleep apnea. ResultsOur models demonstrated significant predictive capability for several outcomes, including age (R2 = 0.643, p < 0.001), and sex classification (area under the receiver operator characteristic (AUROC) curve = 0.808), diabetes and hypertension diagnosis (AUROC = 0.832 and 0.755, respectively). Significant predictions were also modeled for metabolic/endocrine functions (including blood concentrations of IGF-1, leptin, ghrelin, adiponectin, and glucose), and immune markers (including IL-6, TNF-alpha, and CRP). In addition, this approach provided successful predictions in regression modeling of BMI and both regression and classification of sleep apnea. DiscussionThis study demonstrates the potential of using features from oscillatory events in single-channel sleep EEG as digital biomarkers. These biomarkers can identify key health and demographic factors that both affect brain health and are indicative of core brain functions. By capturing the complex interactions of neural, metabolic, endocrine, and immune systems during sleep, our findings support the development of single-channel EEG as a practical tool for monitoring complex biological processes through metrics that originate in brain physiology. Future research should aim to refine these digital biomarkers for broader home-based applications that may utilize inexpensive "wearable" devices to provide a scalable and accessible tool for tracking brain health-related outcomes.

neuroscience↗

bioRxiv2023

Mapping Sleep's Oscillatory Events as a Biomarker of Alzheimer's Disease

ObjectiveMemory-associated neural circuits produce oscillatory events within single-channel sleep electroencephalography (EEG), including theta bursts (TBs), sleep spindles (SPs) and multiple subtypes of slow waves (SWs). Changes in the temporal "coupling" of these events are proposed to serve as a biomarker for early stages of Alzheimers disease (AD) pathogenesis. MethodsWe analyzed data from 205 aging adults, including single-channel sleep EEG, cerebrospinal fluid (CSF) AD-associated biomarkers, and Clinical Dementia Rating(R) (CDR(R)) scale. Individual SW events were sorted into high and low transition frequencies (TF) subtypes. We utilized time-frequency spectrogram locations within sleep EEG to "map" the precision of SW-TB and SW-SP neural circuit coupling in relation to amyloid positivity (by CSF A{beta}42/A{beta}40 threshold), cognitive impairment (by CDR), and CSF levels of AD-associated biomarkers. ResultsCognitive impairment was associated with lower TB spectral power in both high and low TF SW-TB coupling (p<0.001, p=0.001). Cognitively unimpaired, amyloid positive aging adults demonstrated lower precision of the neural circuits propagating high TF SW-TB (p<0.05) and low TF SW-SP (p<0.005) event coupling, compared to cognitively unimpaired amyloid negative individuals. Biomarker correlations were significant for high TF SW-TB coupling with CSF A{beta}42/A{beta}40 (p=0.005), phosphorylated-tau181 (p<0.005), and total-tau (p<0.05). Low TF SW-SP coupling was also correlated with CSF A{beta}42/A{beta}40 (p<0.01). InterpretationLoss of integrity in neural circuits underlying sleep-dependent memory processing can be measured for both SW-TB and SW-SP coupling in spectral time-frequency space. Breakdown of sleeps memory circuit integrity is associated with amyloid positivity, higher levels of AD-associated pathology, and cognitive impairment.

neuroscience↗

bioRxiv2022

The Rise and Fall of Slow Wave Tides: Vacillations of Slow Wave/Spindle Coupling Shift the Composition of Slow Wave Activity Through Sleep Cycles in Accordance with Depth of Sleep

Slow wave activity (SWA) during sleep is associated with synaptic regulation and memory processing functions. Each cycle of non-rapid-eye-movement (NREM) sleep demonstrates a waxing and waning amount of SWA during the transitions between stages N2 and N3 sleep, and the deeper N3 sleep is associated with an increased density of SWA. Further, SWA is an amalgam of different types of slow waves, each identifiable by their temporal coupling to spindle subtypes with distinct physiological features. The objectives of this study were to better understand the neurobiological properties that distinguish different slow wave and spindle subtypes, and to examine the composition of SWA across cycles of NREM sleep. We further sought to explore changes in the composition of NREM cycles that occur among aging adults. To address these goals, we analyzed subsets of data from two well-characterized cohorts of healthy adults: 1) The DREAMS Subjects Database (n=20), and 2) The Cleveland Family Study (n=60). Our analyses indicate that slow wave/spindle coupled events can be characterized as frontal versus central in their relative distribution between electroencephalography (EEG) channels. The frontal predominant slow waves are identifiable by their coupling to late-fast spindles and occur more frequently during stage N3 sleep. Conversely, the central-associated slow waves are identified by coupling to early-fast spindles and favor occurrence during stage N2 sleep. Together, both types of slow wave/spindle coupled events form the composite of SWA, and their relative contribution to the SWA rises and falls across cycles of NREM sleep in accordance with depth of sleep. Exploratory analyses indicated that older adults produce a different composition of SWA, with a shift toward the N3, frontal subtype, which becomes increasingly predominant during cycles of NREM sleep. Overall, these data demonstrate that subtypes of slow wave/spindle events have distinct cortical propagation patterns and differ in their distribution across lighter versus deeper NREM sleep. Future efforts to understand how slow wave sleep and slow wave/spindle coupling impact memory performance and neurological disease may benefit from examining the composition of SWA to avoid potential confounds that may occur when comparing dissimilar neurophysiological events.

neuroscience↗