Biology subjects

Khatri, H.

Publications and source records attributed to Khatri, H..

3 recordsLinked to original sources

bioRxiv2025

Protocol for the Synthesis of Green Fluorescence Carbon Quantum Dots and Biological Application

Carbon quantum dots (CQDs) are materials that are made up of particles that are only a few n anometers wide. They have no dimensions and can change their fluorescence qualities. They are also verybiocompatible and have a lot of potential to change the chemistry of their surface. Their eco-friendly synthesis from precursors, like citric acid (CA) and ascorbic acid (AA), provides a sustainable approach to highly fluorescent nanomaterials with promising applications in bioimaging, drug delivery, and other biomedical fields. Green-emitting carbon quantum dots (GCQDs) were synthesized via a simple reflux approach using citric acid and ascorbic acid as biomolecular precursors in a solvent solution of ethanol-water (1:2). The obtained GCQDs solution was purified using a dialysis process. Besides this, pH maintenance, lyophilization, and further submission for detailed physicochemical evaluation and characterization are done. The spectroscopic analysis, including UV-visible spectroscopy, fluorescence spectroscopy, and FTIR, confirmed the presence of surface functional groups and strong fluorescence properties and quantum yield calculations of the synthesized GCQDs. Microscopic and structural analyses using XRD, AFM, and TEM revealed the nanoscale, predominantly spherical morphology of the synthesized GCQDs. In addition, in vitro biological evaluations such as MTT assay and cellular uptake analysis were undertaken to evaluate cytocompatibility and intracellular distribution. We study the endocytosis pathway of these GCQDs, with size variations ranging from 3 to 5 nm, in mouse tissue-derived primary cells, tissues, and zebrafish embryos. GCQDs were internalized into mouse kidney and liver primary cells through a clathrin-mediated pathway. The findings confirmed that the produced GCQDs exhibit good water solubility, favorable biocompatibility, and significant potential as candidates for biomedical imaging applications.

bioengineering↗

bioRxiv2025

Protocol for green synthesis of excellent fluorescent mango leaves-derived carbon for advanced theragnostic in biological applications

Carbon quantum dots (CQDs) are zero-dimensional fluorescence nanoparticles that are less than 10 nm in size. They have unique properties like tunable surface functionality, biocompatibility, fluorescence, and low toxicity. This study outlines a detailed protocol for synthesizing CQDs from mango leaf powder using a reflux method at 160 C and 400 rpm for a duration of two hours. The synthesized CQDs were comprehensively characterized using the following techniques, including UV-visible spectroscopy, fluorescent spectrophotometry, atomic force microscopy, dynamic light scattering and zeta potential measurement, Fourier transform infrared spectroscopy, and scanning electron microscopy. In vitro analysis was conducted through an MTT assay, revealing that the CQDs exhibit reduced toxicity in RPE1 cells compared to the control group. Additionally, a concentration-dependent cellular uptake assay was performed to further evaluate the biological performance of the CQDs. The findings suggest the potential of CQDs as safe and effective nanomaterials for various biological applications, including drug delivery and bioimaging.

bioengineering↗

bioRxiv2020

Fine-scale landscape genetics unveiling contemporary asymmetric movement of red panda (Ailurus fulgens) in Kangchenjunga landscape, India

Wildlife management in rapid changing landscapes requires critical planning through cross cutting networks, and understanding of landscape features, often affected by the anthropogenic activities. The present study demonstrates fine-scale spatial patterns of genetic variation and contemporary gene flow of red panda (Ailurus fulgens) populations with respect to landscape connectivity in Kangchenjunga Landscape (KL), India. The study found about 1309.54 Km2 area suitable for red panda in KL-India, of which 62.21% area fell under the Protected Area network. We identified 24 unique individuals from 234 feces collected at nine microsatellite loci. The spatially explicit and non-explicit Bayesian clustering algorithms evident to exhibit population structuring and supported red panda populations to exist in meta-population frame work. In concurrence to the habitat suitability and landscape connectivity models, gene flow results supported a contemporary asymmetric movement of red panda by connecting KL- India in a crescent arc. We demonstrate the structural-operational connectivity of corridors in KL-India that facilitated red panda movement in the past. We also seek for cooperation in Nepal, Bhutan and China to aid in preparing for a comprehensive monitoring plan for the long-term conservation and management of red panda in trans-boundary landscapes.

genetics↗