Keyzers, R. | Search bioRxiv

bioRxiv2026

New Zealand spider orchid (Corybas trilobus) exploits the reproductive behaviour of fungus gnats (Diptera: Mycetophila) for pollination.

Due to a reduce pollinator diversity, plant-pollinator interactions in remote habitats are commonly unspecialised. In New Zealand (NZ), many plants exhibit a generalist pollination syndrome where flowers are open-access and pollinated by a range of insects. Orchids in the genus Corybas seem to be the exception. It is believed they mimic mushrooms and their pollination occur by brood-site deception. This study aims to uncover whether this pollination strategy operates in a form of Corybas trilobus known by the tag-name Corybas Remutaka. We used hand-pollination experiments to determine the breeding system of this orchid. SEM observations and neutral red staining were used to study the floral micro-morphology and location of scent glands, respectively. Pollinators behaviour was observed, and filmed, in the field and ex situ. Olfactory signals were assessed using solid-phase micro-extraction and solvent extractions, and visual cues by photography with a UV sensor. Only hand self- and cross-pollinated flowers formed capsules, while natural fruit-set was low. Male and female individuals of the endemic fungus gnat Mycetophila phyllura (Diptera) were the only pollinators observed. Both sexes can remove and deposit pollinia. None of the typical fungal compounds, or other compounds linked to brood-site deception, were detected in the orchid but a prevalence of alkane, sesquiterpene and fatty aldehydes. Some of these compounds were also detected in a mushroom used by fungus gnats for mating and ovipositioning. Features in the labellum surface and base (e.g. exit holes) and UV reflectance may help directing movement of the gnats within the flower. Corybas Remutaka is a self-compatible, nectarless, pollinator-dependent orchid that relies on a mix of volatiles and micro-morphological features to attract a specific pollinator. The orchid exploits the reproductive instinct of male and female fungus gnats to secure its pollination. This deceptive pollination strategy combines features of rendezvous site pollination and brood-site deception.

plant biology↗

bioRxiv2025

An atlas of microtubule lattice parameters regulated through ligand binding to the microtubule stabilizing sites.

Microtubules (MTs) are dynamic cytoskeletal polymers whose lattice architecture regulates force generation, nucleotide hydrolysis, and recognition by motor proteins and microtubule-associated proteins (MAPs). Microtubule-stabilizing agents (MSAs), including taxanes and laulimalide/peloruside-site ligands, suppress depolymerization by binding to defined lattice sites, yet stabilization is not structurally neutral. How ligand chemistry reshapes lattice organization and function remains unresolved. Here, we address three mechanistic questions. First, do distinct ligand classes induce defined lattice states? Using X-ray fiber diffraction, we show that MSAs selectively stabilize two preferred longitudinal conformations, a compact state ([~]4.06 nm monomer rise) and an expanded state ([~]4.17 nm), while modulating lateral organization reflected in shifts in mean MT radius. These axial spacings cluster around discrete values across chemotypes, indicating stabilization of pre-existing conformational minima rather than continuous distortion. Second, are these states interconvertible upon changes in ligand occupancy? Time-resolved diffraction reveals that longitudinal transitions occur within seconds of ligand addition even at substoichiometric occupancy; whereas, lateral equilibration proceeds slower, consistent with redistribution within heterogeneous protofilament organizations. Third, do such structural states alter nucleotide hydrolysis and motor/MAP behavior? Expanded lattices are associated with reduced apparent GTP hydrolysis rates under steady-state assembly conditions and altered kinesin motility, whereas compact lattices preferentially promote tau binding and distinct motor interaction profiles. Together, these findings establish longitudinal lattice conformation as a regulatory parameter and position microtubule-stabilizing agents as chemical tools that bias a dynamic structural landscape with predictable catalytic and transport consequences. Significance statementMicrotubules generate force and support intracellular transport through lattice geometries selectively recognized by motor proteins and microtubule-associated proteins. Microtubule-stabilizing drugs such as taxanes and epothilones are widely used in chemotherapy but can cause neurotoxicity, likely because stabilization alters lattice architecture rather than simply preventing depolymerization. Here, we show that stabilizing ligands bias microtubules between two preferred longitudinal conformations, compact and expanded, that can switch within seconds of binding, while lateral organization adjusts slower. These structural states differentially regulate GTP hydrolysis and recognition by tau and kinesin, linking lattice geometry to catalytic and transport functions. By establishing lattice conformation as a tunable regulatory parameter, this work provides a framework for interpreting drug effects and designing structure-selective stabilizers with improved therapeutic profiles.

biophysics↗