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Kerby, J. T.

Publications and source records attributed to Kerby, J. T..

2 recordsLinked to original sources

Vegetation monitoring using multispectral sensors - best practices and lessons learned from high latitudes

Emerging drone technologies have the potential to revolutionise ecological monitoring. The rapid technological advances in recent years have dramatically increased affordability and ease of use of Unmanned Aerial Vehicles (UAVs) and associated sensors. Compact multispectral sensors, such as the Parrot Sequoia (Paris, France) and MicaSense RedEdge (Seattle WA, USA) capture spectrally accurate high-resolution (fine grain) imagery in visible and near-infrared parts of the electromagnetic spectrum, providing supplement to satellite and aircraft-based imagery. Observations of surface reflectance can be used to calculate vegetation indices such as the Normalised Difference Vegetation Index (NDVI) for productivity estimates and vegetation classification. Despite the advances in technology, challenges remain in capturing consistently high-quality data, particularly when operating in extreme environments such as the high latitudes. Here, we summarize three years of ecological monitoring with drone-based multispectral sensors in the remote Canadian Arctic. We discuss challenges, technical aspects and practical considerations, and highlight best practices that emerged from our experience, including: flight planning, factoring in weather conditions, and geolocation and radiometric calibration. We propose a standardised methodology based on established principles from remote sensing and our collective field experiences, using the Parrot Sequoia sensor as an example. With these good practises, multispectral sensors can provide meaningful spatial data that is reproducible and comparable across space and time.

ecology

Investigating the viral ecology of global bee communities with high-throughput metagenomics

Bee viral ecology is a fascinating emerging area of research: viruses exert a range of effects on their hosts, exacerbate the impacts of other environmental stressors, and, importantly, are readily shared across multiple bee species in a community. However, our understanding of bee viral communities is limited, as it is primarily derived from studies of North American and European Apis mellifera populations. Here, we examined viruses in populations of A. mellifera and 11 other bee species from 9 countries, across 5 continents and Oceania. We developed a novel pipeline to rapidly, inexpensively, and robustly screen for bee viruses. This pipeline includes purification of encapsulated RNA/DNA viruses, sequence-independent amplification, high throughput sequencing, integrated assembly of contigs, and filtering to identify contigs specifically corresponding to viral sequences. We identified sequences corresponding to (+)ssRNA, (-)ssRNA, dsRNA, and ssDNA viruses. Overall, we found 127 contigs corresponding to novel viruses (i.e. previously not observed in bees), with 29 represented by >0.1 % of the reads in a given sample. These viruses and viral families were distributed across multiple regions and species. This study provides a robust pipeline for metagenomics analysis of viruses, and greatly expands our understanding of the diversity of viruses found in bee communities.

genomics